Sirtuin 1 regulates pulmonary artery smooth muscle cell proliferation: role in pulmonary arterial hypertension.

J Hypertens 2018 05;36(5):1164-1177

INSERM UMR-S 1180, Université Paris-Sud, Université Paris-Saclay, Faculté de Pharmacie, Châtenay-Malabry.

Objective: Energy metabolism shift from oxidative phosphorylation toward glycolysis in pulmonary artery smooth muscle cells (PASMCs) is suggested to be involved in their hyperproliferation in pulmonary arterial hypertension (PAH). Here, we studied the role of the deacetylase sirtuin1 (SIRT1) in energy metabolism regulation in PASMCs via various pathways including activation of peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1α), master regulator of mitochondrial biogenesis.

Approach And Results: Contents of PGC-1α and its downstream targets as well as markers of mitochondrial mass (voltage-dependent anion channel and citrate synthase) were diminished in human PAH PASMCs. These cells and platelet-derived growth factor-stimulated rat PASMCs demonstrated a shift in cellular acetylated/deacetylated state, as evidenced by the increase of the acetylated forms of SIRT1 targets: histone H1 and Forkhead box protein O1. Rat and human PASMC proliferation was potentiated by SIRT1 pharmacological inhibition or specific downregulation via short-interfering RNA. Moreover, after chronic hypoxia exposure, SIRT1 inducible knock out mice displayed a more intense vascular remodeling compared with their control littermates, which was associated with an increase in right ventricle pressure and hypertrophy. SIRT1 activator Stac-3 decreased the acetylation of histone H1 and Forkhead box protein O1 and strongly inhibited rat and human PASMC proliferation without affecting cell mortality. This effect was associated with the activation of mitochondrial biogenesis evidenced by higher expression of mitochondrial markers and downstream targets of PGC-1α.

Conclusion: Altered acetylation/deacetylation balance as the result of SIRT1 inactivation is involved in the pathogenesis of PAH, and this enzyme could be a promising therapeutic target for PAH treatment.

Download full-text PDF

Source
http://dx.doi.org/10.1097/HJH.0000000000001676DOI Listing
May 2018
147 Reads

Publication Analysis

Top Keywords

downstream targets
8
smooth muscle
8
rat human
8
human pasmc
8
arterial hypertension
8
pasmc proliferation
8
pulmonary arterial
8
energy metabolism
8
artery smooth
8
histone forkhead
8
pulmonary artery
8
forkhead box
8
box protein
8
sirt1
6
potentiated sirt1
4
targets histone
4
protein rat
4
proliferation potentiated
4
inhibition specific
4
hypoxia exposure
4

Similar Publications