Cytokine- and TCR-Mediated Regulation of T Cell Expression of Ly6C and Sca-1.

J Immunol 2018 03 22;200(5):1761-1770. Epub 2018 Jan 22.

Department of Pathobiology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, PA 19104;

Ly6C and Sca-1 (Ly6A/E) are Ly6 family GPI-anchored surface molecules that are differentially expressed by multiple immune populations. Ly6C expression has been used to distinguish short-lived effector CD4 T cells from memory precursor effector cells, whereas Sca-1 has been used in the identification of CD8 memory stem cells. This study examines the expression patterns of these molecules and establishes that, in vitro, IL-27, type I IFN, and IFN-γ are potent inducers of Ly6C and Sca-1 in naive mouse CD4 and CD8 T cells, whereas TGF-β limits their expression. The induction of Ly6C and Sca-1 by IL-27 and IFN-γ is dependent on STAT1, but not STAT3 or T-bet. In mouse splenocytes, at homeostasis, Ly6C and Sca-1 expression was not restricted to effector cells, but was also found at various levels on naive and memory populations. However, in response to infection with , pathogen-specific T cells expressed high levels of these molecules and in this context, endogenous IL-27 and IFN-γ were required for the expression of Ly6C but not Sca-1. Together, these findings highlight the TCR-dependent and cytokine-mediated signals that modulate T cell expression of Ly6C and Sca-1 in vitro and in vivo during infection.

Download full-text PDF

Source
http://dx.doi.org/10.4049/jimmunol.1701154DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5821564PMC
March 2018
5 Reads

Publication Analysis

Top Keywords

ly6c sca-1
28
expression ly6c
12
ly6c
8
effector cells
8
cell expression
8
il-27 ifn-γ
8
sca-1
8
expression
7
cells
6
sca-1 il-27
4
induction ly6c
4
expression induction
4
tgf-β limits
4
limits expression
4
splenocytes homeostasis
4
ifn-γ dependent
4
stat1 stat3
4
stat3 t-bet
4
t-bet mouse
4
mouse splenocytes
4

Similar Publications