A Comprehensive Protocol Resource for Performing Pooled shRNA and CRISPR Screens.

Methods Mol Biol 2018 ;1725:201-227

Victorian Centre for Functional Genomics, Peter MacCallum Cancer Centre, Melbourne, VIC, Australia.

This chapter details a compendium of protocols that collectively enable the reader to perform a pooled shRNA and/or CRISPR screen-with methods to identify and validate positive controls and subsequent hits; establish a viral titer in the cell line of choice; create and screen libraries, sequence strategies, and bioinformatics resources to analyze outcomes. Collectively, this provides an overarching resource from the start to finish of a screening project, making this technology possible in all laboratories.

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http://dx.doi.org/10.1007/978-1-4939-7568-6_17DOI Listing
January 2019
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