The Long Noncoding RNA Is Involved in the Regulation of Cell Proliferation.

Anticancer Res 2018 01;38(1):77-81

Graduate School of Humanity-Oriented Science and Engineering, Faculty of Humanity-Oriented Science and Engineering, Kindai University, Iizuka, Japan

Background/aim: OPA-interacting protein 5 antisense transcript 1 (OIP5-AS1) is a long noncoding RNA located on human chromosome 15q15.1 and transcribed in the opposite direction to OIP5. Here, we report that OIP5-AS1 is involved in regulating cell proliferation.

Materials And Methods: HeLa cells were transfected with OIP5-AS1-targeting siRNA oligonucleotides and anti-sense oligonucleotides. The cells were harvested 72 h after transfection and subjected to quantitative reverse transcription-polymerase chain reaction (qRT-PCR), and cell-cycle and apoptosis analysis.

Results: OIP5-AS1 was expressed at a lower level in cells harbouring an oncogenic kirsten rat sarcoma viral oncogene homolog (K-RAS) mutation than in cells expressing wild-type K-RAS. Silencing OIP5-AS1 with siRNA oligonucleotides or anti-sense oligonucleotides reduced HeLa cell proliferation. Apoptosis and cell-cycle analysis showed that silencing OIP5-AS1 did not cause apoptosis, but did cause G/M phase cell-cycle arrest.

Conclusion: These results suggest that OIP5-AS1 positively regulates cell proliferation by promoting G/M phase progression.

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http://dx.doi.org/10.21873/anticanres.12194DOI Listing
January 2018
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