Search our Database of Scientific Publications and Authors

I’m looking for a

    Details and Download Full Text PDF:
    Effect of ECM2 expression on bovine skeletal muscle-derived satellite cell differentiation.

    • Authors:
    • Chang Liu
      The First Affiliated Hospital of Xi'an Jiaotong University
      Xian Shi | China
      Huili Tong
      Northeast Agricultural University
      China
      Shufeng Li
      Nanjing University
      China
      Yunqin Yan
      Northeast Agricultural University
      China
    Cell Biol Int 2018 May 27;42(5):525-532. Epub 2018 Feb 27.
    The Laboratory of Cell and Development, Northeast Agricultural University, Changjiang Road No. 600, Xiang Fang Strict, Harbin, Heilongjiang, 150030, China.
    Extracellular matrix components have important regulatory functions during cell proliferation and differentiation. In recent study, extracellular matrix were shown to have a strong effect on skeletal muscle differentiation. Here, we aimed to elucidate the effects of extracellular matrix protein 2 (ECM2), an extracellular matrix component, on the differentiation of bovine skeletal muscle-derived satellite cells (MDSCs). Western blot and immunofluorescence analyses were used to elucidate the ECM2 expression pattern in bovine MDSCs during differentiation in vitro. CRISPR/Cas9 technology was used to activate or inhibit ECM2 expression to study its effects on the in vitro differentiation of bovine MDSCs. ECM2 expression was shown to increase gradually during bovine MDSC differentiation, and the levels of this protein were higher in more highly differentiated myotubes. ECM2 activation promoted MDSC differentiation, whereas its suppression inhibited the differentiation of these cells. Here, for the first time, we demonstrated the importance of ECM2 expression during bovine MDSC differentiation; these results could lead to treatments that help to increase beef cattle muscularity.
    PDF Download - Full Text Link
    ( Please be advised that this article is hosted on an external website not affiliated with PubFacts.com)
    Source Status
    http://dx.doi.org/10.1002/cbin.10927DOI ListingPossible

    Similar Publications

    Effect of ELOVL3 expression on bovine skeletal muscle-derived satellite cell differentiation.
    Biochem Biophys Res Commun 2017 12 3;493(4):1457-1463. Epub 2017 Aug 3.
    The Laboratory of Cell and Development, Northeast Agricultural University, Harbin, Heilongjiang 150030, China. Electronic address:
    ELOVL3 is involved in elongating saturated and monounsaturated fatty acids, and is a critical enzyme for lipid accumulation in brown adipocytes during the early phase of tissue recruitment. In addition, ELOVL3 is related to increased fatty acid oxidation in brown adipocytes. However, the potential functions of ELOVL3 in bovine cells remain unclear. Read More
    Effect of TCEA3 on the differentiation of bovine skeletal muscle satellite cells.
    Biochem Biophys Res Commun 2017 03 2;484(4):827-832. Epub 2017 Feb 2.
    The Laboratory of Cell and Development, Northeast Agricultural University, Harbin, Heilongjiang 150030, China. Electronic address:
    Bovine muscle-derived satellite cells (MDSCs) are important for animal growth. In this study, the effect of transcription elongation factor A3 (TCEA3) on bovine MDSC differentiation was investigated. Western blotting, immunofluorescence assays, and cytoplasmic and nuclear protein isolation and purification techniques were used to determine the expression pattern and protein localization of TCEA3 in bovine MDSCs during in vitro differentiation. Read More
    Transcription factor EGR1 promotes differentiation of bovine skeletal muscle satellite cells by regulating MyoG gene expression.
    J Cell Physiol 2018 Jan 18;233(1):350-362. Epub 2017 May 18.
    The Laboratory of Cell and Development, Northeast Agricultural University, XiangFang District, Harbin, Heilongjiang Province, China.
    The transcription factor, early growth response 1 (EGR1), has important roles in various cell types in response to different stimuli. EGR1 is thought to be involved in differentiation of bovine skeletal muscle-derived satellite cells (MDSCs); however, the precise effects of EGR1 on differentiation of MDSCs and its mechanism of action remain unknown. In the present study, a time course of EGR1 expression and the effects of EGR1 on MDSC differentiation were determined. Read More
    Effect of differentiation on microRNA expression in bovine skeletal muscle satellite cells by deep sequencing.
    Cell Mol Biol Lett 2016 28;21. Epub 2016 Jul 28.
    The Key Laboratory of Mammal Reproductive Biology and Biotechnology Ministry of Education, Inner Mongolia University, Hohhot, 010021 China.
    Background: The differentiation of skeletal muscle-derived satellite cells (MDSCs) is important in controlling muscle growth, improving livestock muscle quality, and healing of muscle-related disease. MicroRNAs (miRNAs) are a class of gene expression regulatory factors, which play critical roles in the regulation of muscle cell differentiation. This study aimed to compare the expression profile of miRNAs in MDSC differentiation, and to investigate the miRNAs which are involved in MDSC differentiation. Read More