PKM2 activation sensitizes cancer cells to growth inhibition by 2-deoxy-D-glucose.

Oncotarget 2017 Oct 26;8(53):90959-90968. Epub 2017 Jul 26.

Department of Radiology, Stanford University, Stanford, CA, USA.

Cancer metabolism has emerged as an increasingly attractive target for interfering with tumor growth. Small molecule activators of pyruvate kinase isozyme M2 (PKM2) suppress tumor formation but have an unknown effect on established tumors. We demonstrate that TEPP-46, a PKM2 activator, results in increased glucose consumption, providing the rationale for combining PKM2 activators with the toxic glucose analog, 2-deoxy-D-glucose (2-DG). Combination treatment resulted in reduced viability of a range of cell lines in standard cell culture conditions at concentrations of drugs that had no effect when used alone. This effect was replicated on established subcutaneous tumors. We further demonstrated the ability to detect acute metabolic differences in combination treatment using hyperpolarized magnetic resonance spectroscopy (MRS). Combination treated tumors displayed a higher pyruvate to lactate C-label exchange 2 hr post-treatment. This ability to assess the effect of drugs non-invasively may accelerate the implementation and clinical translation of drugs that target cancer metabolism.

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Source
http://dx.doi.org/10.18632/oncotarget.19630DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5710897PMC
October 2017
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