ATM Is Required for the Prolactin-Induced HSP90-Mediated Increase in Cellular Viability and Clonogenic Growth After DNA Damage.

Authors:
Florence Boutillon
Florence Boutillon
PRL/GH Pathophysiology Laboratory
Vincent Goffin
Vincent Goffin
Research Center Growth and Signaling
Carrie S Shemanko
Carrie S Shemanko
University of Calgary
Canada

Endocrinology 2018 02;159(2):907-930

Department of Biological Sciences, University of Calgary, Calgary, Alberta, Canada.

Prolactin (PRL) acts as a survival factor for breast cancer cells, but the PRL signaling pathway and the mechanism are unknown. Previously, we identified the master chaperone, heat shock protein 90 (HSP90) α, as a prolactin-Janus kinase 2 (JAK2)-signal transducer and activator of transcription 5 (STAT5) target gene involved in survival, and here we investigated the role of HSP90 in the mechanism of PRL-induced viability in response to DNA damage. The ataxia-telangiectasia mutated kinase (ATM) protein plays a critical role in the cellular response to double-strand DNA damage. We observed that PRL increased viability of breast cancer cells treated with doxorubicin or etoposide. The increase in cellular resistance is specific to the PRL receptor, because the PRL receptor antagonist, Δ1-9-G129R-hPRL, prevented the increase in viability. Two different HSP90 inhibitors, 17-allylamino-17-demethoxygeldanamycin and BIIB021, reduced the PRL-mediated increase in cell viability of doxorubicin-treated cells and led to a decrease in JAK2, ATM, and phosphorylated ATM protein levels. Inhibitors of JAK2 (G6) and ATM (KU55933) abolished the PRL-mediated increase in cell viability of DNA-damaged cells, supporting the involvement of each, as well as the crosstalk of ATM with the PRL pathway in the context of DNA damage. Drug synergism was detected between the ATM inhibitor (KU55933) and doxorubicin and between the HSP90 inhibitor (BIIB021) and doxorubicin. Short interfering RNA directed against ATM prevented the PRL-mediated increase in cell survival in two-dimensional cell culture, three-dimensional collagen gel cultures, and clonogenic cell survival, after doxorubicin treatment. Our results indicate that ATM contributes to the PRL-JAK2-STAT5-HSP90 pathway in mediating cellular resistance to DNA-damaging agents.

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February 2018
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References

(Supplied by CrossRef)
What is breast in the bone
Shemanko et al.
Int J Mol Sci 2016
Efficacy of monochemotherapy with docetaxel (Taxotere) in relation to prolactin secretion in heavily pretreated metastatic breast cancer
Lissoni et al.
Neuroendocrinol Lett 2001
Importance of serum prolactin determination in metastatic breast cancer patients
Mujagić et al.
Croat Med J 2004

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