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    Expression of DCUN1D1 in laryngeal squamous cell carcinoma and its inhibiting effect on TU-177 cells after interfered by RNA.
    • Authors:
    • Jing Liu
      Capital Medical University
      China
      Yu Shuang
      the Second Affiliated Hospital of Tianjin Medical University
      China
      Chao Li
      Auburn University
      United States
      Xuan Zhou
      College of Veterinary Medicine
      China
      Yongwang Huang
      Second Hospital of Tianjin Medical University
      Lun Zhang
      Chengdu University of Traditional Chinese Medicine
      China
    Clin Exp Pharmacol Physiol 2017 Nov 22. Epub 2017 Nov 22.
    Department of Otorhinolaryngology and Maxillofacial Oncology, Tianjin Medical University Cancer Institute, Hospital, Key Laboratory of Cancer Prevention and Therapy, Tianjin Cancer Institute; National Clinical Research Center of Cancer;, Tianjin.
    Expression of DCUN1D1 in laryngeal squamous cell carcinoma(LSCC) and its inhibition by small interfering RNA(siRNA) target in the TU-177 cells was investigated. Immunohistochemistry was used to detect the expression level of DCUN1D1 in LSCC tissue in 140 cases and analyze its relationship with clinical pathological characteristics. siRNA expression plasmid targeting DCUN1D1 was constructed and transferred into TU-177 cells. The effect of siRNA target DCUN1D1 gene silencing on proliferation, invasion and migration of TU-177 cells were observed by MTS assay and Transwell experiment. The expression levels of Focal adhesion kinase (FAK) and matrix metalloproteinase-2(MMP-2) were detected by Western blot. Expression level of DCUN1D1 protein increased significantly in T3+T4, N+, and III+IV stages of LSCC patients (P<0.05). After DCUN1D1 was targeted by siRNA, the DCUN1D1 protein level decreased 67% in siRNA-3 group, where average absorbance value was lower than the control and blank group with significant difference(F=6.076,P<0.05) in MTS assay, meantime migration, and invasion cells in each vision were the same (F=19.851, F=25.454, P<0.01) in Transwell experiment. The expression level of FAK and MMP-2 was significantly down-regulated in siRNA-3 group(F=28.896, F=40.240, P<0.01). DCUN1D1 is associated with progression and prognosis of LSCC. After siRNA based target on DCUN1D1, TU-177 cells growth was inhibited and invasion of malignant tumor was diminished by reducing the expression of FAK and MMP-2. DCUN1D1 is could become a potential new target for the treatment of LSCC. This article is protected by copyright. All rights reserved.

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