Effect of a rare genetic variant of TM7SF4 gene on osteoclasts of patients with Paget's disease of bone.

Authors:
Emilie Laurier
Emilie Laurier
CHU de Québec-Université Laval Research Centre
Québec | Canada
Nathalie Amiable
Nathalie Amiable
Notre-Dame Hospital
Canada
Edith Gagnon
Edith Gagnon
Laval University
Canada
Jacques P Brown
Jacques P Brown
McMaster University
Canada
Laetitia Michou
Laetitia Michou
Faculté de Médecine de l'Université Laval

BMC Med Genet 2017 11 16;18(1):133. Epub 2017 Nov 16.

CHU de Québec-Université Laval Research Centre, Quebec, QC, Canada.

Background: Dendritic Cell-Specific Transmembrane Protein (DC-STAMP) is involved in osteoclastogenesis with a key role in mononucleated osteoclasts fusion. We reported in patients with Paget's disease of bone (PDB) a rare variant (rs62620995) in the TM7SF4 gene, encoding for DC-STAMP, which changes a highly conserved amino acid, possibly damaging according to in silico predictions. This study aimed at determining the functional effects of this variant on osteoclast phenotype in PDB.

Methods: Fifty ml of peripheral blood were collected in pagetic patients carrier of this variant (n = 4) or not (n = 4) and healthy controls (n = 4). Monocytes were collected after Ficoll gradient and cultured in a medium containing RANKL (40 ng/ml) and hMCSF (25 ng/ml). At the end of the differentiation period, we assessed the osteoclast morphology and bone resorption abilities. We quantified gene expression of SQSTM1, DC-STAMP, OS9, CREB3, LAMP1, OC-STAMP, and NFATC1 genes from cell lysates. Proteins encoded by these genes were investigated by Western Blot. Statistical analyses relied on ANOVA followed by Tukey post-tests.

Results: After 21 days of differentiation, the mean number of nuclei per multinucleated cell was significantly higher in pagetic patients carrier of the variant than in healthy controls. Bone resorption abilities were not modified by the variant. qPCR and Western Blot analyses did not provide any differences, but DC-STAMP expression was higher in patients carrier of the variant than in patients non carrier.

Conclusions: This TM7SF4 rare variant may have an impact on osteoclast morphology and on DC-STAMP expression during osteoclastogenesis. Further analyses are required to understand the role of this variant during osteoclastogenesis in PDB.

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Source
http://dx.doi.org/10.1186/s12881-017-0495-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5693359PMC
November 2017
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