Gut Microbiota Interacts with Markers of Adipose Tissue Browning, Insulin Action and Plasma Acetate in Morbid Obesity.

Mol Nutr Food Res 2018 02 29;62(3). Epub 2017 Dec 29.

Department of Diabetes, Endocrinology and Nutrition, Institut d'Investigació Biomèdica de Girona (IdIBGi), CIBEROBN (CB06/03/010) and Instituto de Salud Carlos III (ISCIII), Girona, Spain.

Scope: To examine the potential relationship among gene expression markers of adipose tissue browning, gut microbiota, and insulin sensitivity in humans.

Methods And Results: Gut microbiota composition and gene markers of browning are analyzed in subcutaneous (SAT) and visceral (VAT) adipose tissue from morbidly obese subjects (n = 34). Plasma acetate is measured through H NMR and insulin sensitivity using euglycemic hyperinsulinemic clamp. Subjects with insulin resistance show an increase in the relative abundance (RA) of the phyla Bacteroidetes and Proteobacteria while RA of Firmicutes is decreased. In all subjects, Firmicutes RA is negatively correlated with HbA and fasting triglycerides, whereas Proteobacteria RA was negatively correlated with insulin sensitivity. Firmicutes RA is positively associated with markers of brown adipocytes (PRDM16, UCP1, and DIO2) in SAT, but not in VAT. Multivariate regression analysis indicates that Firmicutes RA contributes significantly to SAT PRDM16, UCP1, and DIO2 mRNA variance after controlling for age, BMI, HbA , or insulin sensitivity. Interestingly, Firmicutes RA, specifically those bacteria belonging to the Ruminococcaceae family, is positively associated with plasma acetate levels, which are also linked to SAT PRDM16 mRNA and insulin sensitivity.

Conclusion: Gut microbiota composition is linked to adipose tissue browning and insulin action in morbidly obese subjects, possibly through circulating acetate.

Download full-text PDF

Source
http://dx.doi.org/10.1002/mnfr.201700721DOI Listing
February 2018
87 Reads

Publication Analysis

Top Keywords

adipose tissue
16
insulin sensitivity
16
gut microbiota
16
plasma acetate
12
tissue browning
12
negatively correlated
8
morbidly obese
8
sat prdm16
8
microbiota composition
8
ucp1 dio2
8
prdm16 ucp1
8
insulin action
8
positively associated
8
obese subjects
8
insulin
8
markers adipose
8
browning insulin
8
firmicutes
5
abundance phyla
4
markers brown
4

References

(Supplied by CrossRef)

Hamady et al.
Genome Res. 2009

Ursell et al.
Gastroenterology 2014

Tagliabue et al.
Nutr. Metab. Cardiovasc. Dis. 2013

Rosenbaum et al.
Trends Endocrinol. Metab. 2015

Ravussin et al.
Obesity (Silver Spring) 2012

Palleja et al.
Genome Med. 2016

Walters et al.
FEBS Lett. 2014

Chevalier et al.
Cell 2015

Li et al.
Cell Metab. 2017

Cohen et al.
Cell 2014

Kumari et al.
J. Clin. Invest. 2016

Similar Publications