Engineering a synthetic pathway for maleate in Escherichia coli.

Authors:
Shuhei Noda
Shuhei Noda
Biomass Engineering Program
Tomokazu Shirai
Tomokazu Shirai
Osaka University
Japan
Yutaro Mori
Yutaro Mori
Graduate School of Engineering
Sachiko Oyama
Sachiko Oyama
Fukuoka University
Japan
Akihiko Kondo
Akihiko Kondo
Graduate School of Science
Japan

Nat Commun 2017 10 27;8(1):1153. Epub 2017 Oct 27.

Center for Sustainable Resource Science, RIKEN, 1-7-22, Suehiro-cho, Tsurumi-ku, Yokohama, Kanagawa, 230-0045, Japan.

Maleate is one of the most important dicarboxylic acids and is used to produce various polymer compounds and pharmaceuticals. Herein, microbial production of maleate is successfully achieved, to our knowledge for the first time, using genetically modified Escherichia coli. A synthetic pathway of maleate is constructed in E. coli by combining the polyketide biosynthesis pathway and benzene ring cleavage pathway. The metabolic engineering approach used to fine-tune the synthetic pathway drastically improves maleate production and demonstrates that one of the rate limiting steps exists in the conversion of chorismate to gentisate. In a batch culture of the optimised transformant, grown in a 1-L jar fermentor, the amount of produced maleate reaches 7.1 g L, and the yield is 0.221 mol mol. Our results suggest that the construction of synthetic pathways by combining a secondary metabolite pathway and the benzene ring cleavage pathway is a powerful tool for producing various valuable chemicals.

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Environmentally friendly maleate production using genetically modified microorganisms


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http://dx.doi.org/10.1038/s41467-017-01233-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5658364PMC

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October 2017
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