Lewis Rat Model of Experimental Autoimmune Encephalomyelitis.

Curr Protoc Neurosci 2017 Oct 23;81:9.61.1-9.61.20. Epub 2017 Oct 23.

Department of Neurology, St. Josef Hospital, Ruhr-University Bochum, Bochum, Germany.

In this unit, we describe in detail the most common methods used to break immunological tolerance for central myelin antigens and induce experimental autoimmune encephalomyelitis (EAE) in Lewis rats as an animal model of multiple sclerosis. The resulting disease course ranges from an acute monophasic disease to a chronic relapsing or chronic progressive course, which strongly resembles the human disease. These models enable the study of cellular and humoral autoimmunity against major antigenic epitopes of the myelin basic protein, myelin oligodendrocyte glycoprotein, or proteolipid protein. We provide an overview of common immunization protocols for induction of active and passive EAE, assessment and analysis of clinical score, preparation and purification of myelin basic protein, and derivation of neuroantigen-specific rat T cell lines. Finally, we describe the major clinical characteristics of these models. © 2017 by John Wiley & Sons, Inc.

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http://dx.doi.org/10.1002/cpns.36DOI Listing
October 2017
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References

(Supplied by CrossRef)
Basic A1 protein of the myelin membrane. The complete amino acid sequence
Eylar E. H. et al.
The Journal of Biological Chemistry 1971

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