Profiling glucose-induced selective inhibition of disaccharide catabolism in Bacillus megaterium QM B1551 by stable isotope labelling.

Authors:
Tracy Youngster
Tracy Youngster
School of Integrative Plant Science
Ithaca | United States
Julie A Wushensky
Julie A Wushensky
College of Agriculture and Life Sciences
Dr. Ludmilla Aristilde, PhD
Dr. Ludmilla Aristilde, PhD
Cornell University
Associate Professor
Environmental Chemistry; Environmental Biochemistry; Environmental Engineering.
Ithaca, NY | United States

Microbiology 2017 Oct 28;163(10):1509-1514. Epub 2017 Sep 28.

2​Department of Biological and Environmental Engineering, College of Agriculture and Life Sciences, Cornell University, Ithaca, NY 14853, USA.

We investigated the co-catabolism of carbohydrate mixtures in Bacillus megaterium QM B1551 using a C-assisted metabolomics profiling approach. Specifically, we monitored the ability of B. megaterium to achieve the simultaneous catabolism of glucose and a common disaccharide - cellobiose, maltose, or sucrose. Growth experiments indicated that each disaccharide alone can serve as a sole carbon source for B. megaterium, in accordance with the genetic analysis of this bacterium, which predicted diverse metabolic capabilities. However, following growth on C-labelled glucose and each unlabelled disaccharide, the labelling patterns of the intracellular metabolites in glycolysis and the pentose phosphate pathway revealed a hierarchy in disaccharide catabolism: (i) complete inhibition of cellobiose catabolism, (ii) minimal catabolism of maltose and (iii) unbiased catabolism of sucrose. The labelling of amino acids confirmed this selective assimilation of each substrate in biomass precursors. This study highlights the fact that B. megaterium exhibits a mixed-carbohydrate utilization that is different from that of B. subtilis, the most studied model Bacillus species.

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http://dx.doi.org/10.1099/mic.0.000540DOI Listing
October 2017
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(Supplied by CrossRef)

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1996

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