Synergistic effect of spexin and progesterone on pain sensitivity attenuation in ovariectomized rats.

Clin Exp Pharmacol Physiol 2018 Apr 11;45(4):349-354. Epub 2017 Dec 11.

Department of Physiology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran.

Spexin is a central modulator of nociception. The aim of the present study was to investigate the effect of intra-hippocampal CA3 (IHCA3) injection of spexin and spexin-progesterone co-administration on pain sensitivity in ovariectomized rat. Thirty-five adult female rats were divided into five groups. Sham: the animals received injection of 0.5 μL ACSF by IHCA3. Experiments 1 and 2: the animals received injection of 0.5 μL of spexin bilaterally (10 and 30 nmol/rat respectively). Experiments 3 and 4: the animals received injection of 0.5 μL of spexin bilaterally (10 and 30 nmol/rat respectively) + subcutaneous (s.c.) injection of progesterone (5 mg/kg). Ovariectomy was performed in all groups to eliminate the effects of cyclic changes in the female rats. The formalin test (formalin 2.5%) was performed following the administration of spexin and progesterone. Results showed that bilateral injection of spexin in IHCA3 at both concentrations a significant (P < .05) decrease in the pain sensitivity in the two phases of formalin test. Similarly, the bilateral injection of spexin in IHCA3 at both concentrations following the s.c. injection of progesterone significantly (P < .05) decreases pain sensitivity in two phases of the formalin test. This pain attenuation due to the co-administration of spexin and progesterone was more potent than spexin-induced analgesia. According to the present results, spexin has a modulatory effect on pain sensitivity, which becomes more pronounced by progesterone administration.

Download full-text PDF

Source
http://dx.doi.org/10.1111/1440-1681.12862DOI Listing
April 2018
4 Reads

Publication Analysis

Top Keywords

received injection
12
animals received
12
injection 05 μl
12
spexin bilaterally
8
05 μl spexin
8
injection spexin
8
experiments animals
8
pain sensitivity
8
female rats
8
bilaterally 30 nmol/rat
8
spexin progesterone
8
injection
6
spexin
6
respectively + subcutaneous injection
4
30 nmol/rat experiments
4
30 nmol/rat respectively + subcutaneous
4
05 μl acsf
4
rats divided
4
adult female
4
divided groups
4

Similar Publications

Peptides derived from the prohormone proNPQ/spexin are potent central modulators of cardiovascular and renal function and nociception.

FASEB J 2012 Feb 28;26(2):947-54. Epub 2011 Oct 28.

SRI International, Menlo Park, California, USA.

Computational methods have led two groups to predict the endogenous presence of a highly conserved, amidated, 14-aa neuropeptide called either spexin or NPQ. NPQ/spexin is part of a larger prohormone that contains 3 sets of RR residues, suggesting that it could yield more than one bioactive peptide; however, no in vivo activity has been demonstrated for any peptide processed from this precursor. Here we demonstrate biological activity for two peptides present within proNPQ/spexin. Read More

View Article
February 2012

Estradiol and progesterone differentially regulate formalin-induced nociception in ovariectomized female rats.

Horm Behav 2006 Apr 27;49(4):441-9. Epub 2005 Oct 27.

Department of Psychology, Hunter College and The Graduate Center of the City University of New York, 695 Park Avenue, New York, NY 10021, USA.

Clinical and preclinical studies have found sex-specific differences in the discrimination and perception of inflammatory stimuli. The emerging picture suggests that the biological basis of these differences resides in the regulatory activity of gonadal hormones in the central nervous system. This study describes the effects of ovarian hormones in inflammatory pain processes. Read More

View Article
April 2006

The effects of cycling levels of 17beta-estradiol and progesterone on the magnitude of temporomandibular joint-induced nociception.

Endocrinology 2009 Aug 9;150(8):3680-9. Epub 2009 Apr 9.

Department of Biomedical Sciences, Texas A&M Health Science Center, Baylor College of Dentistry, Dallas, Texas 75246, USA.

A greater incidence of temporomandibular joint (TMJ) pain is reported in females, suggesting that gonadal hormones may play a role in this condition. However, the exact roles of 17beta-estradiol (E2) and progesterone (P4) in TMJ pain are not completely known. Two experiments were performed to determine the separate roles of E2 and P4 in TMJ nociception at various stages of the estrous cycle. Read More

View Article
August 2009

Intra-periaqueductal gray matter microinjection of orexin-A decreases formalin-induced nociceptive behaviors in adult male rats.

J Pain 2011 Feb 10;12(2):280-7. Epub 2010 Dec 10.

Department of Physiology, School of Medical Sciences, Tarbiat Modares University, Tehran, Iran.

Unlabelled: Intracerebroventricular injection of orexin-A (hypocretin-1) has been shown to elicit the analgesic responses. However, the locations of central sites that may mediate these effects have not been clearly elucidated. This study was performed using male Sprague Dawley rats to investigate the antinociceptive effects of intra-periaqueductal gray matter (PAG) administration of orexin-A, 5 minutes prior to formalin (50 μL of 2%) injection. Read More

View Article
February 2011