Aspirin-Induced Gastric Lesions Alters EGFR and PECAM-1 Immunoreactivity in Wistar Rats: Modulatory Action of Flavonoid Fraction of .

Authors:
Dr Margaret Olutayo Alese, PhD
Dr Margaret Olutayo Alese, PhD
University of KwaZulu-Natal
Post-doctoral Research Fellow
Durban, KwaZulu-Natal | South Africa

Open Access Maced J Med Sci 2017 Aug 26;5(5):569-577. Epub 2017 Jul 26.

Department of Physiology, College of Medicine, Ekiti State University, Ado Ekiti, Nigeria.

Aim: In this study, Epithelial Growth Factor Receptor and Platelet Endothelial Cell Adhesion Molecule-1 were localised to investigate the healing effects of a flavonoid-rich fraction of fruit in the gastric corpus of Wistar rats following aspirin-induced gastric lesion.

Materials And Methods: Mature, unripe fruits of were peeled; air dried, pulverised, extracted with 70% methanol, concentrated and partitioned. Ninety male Wistar rats were randomly assigned into 6 groups of 15 rats each. The gastric lesion was induced in groups B, C, D, E and F rats by administration of 400 mg/kg aspirin in distilled water. Group A received distilled water. After 24 hours, flavonoid fraction of was administered to groups C, D and E at 100, 200 and 400 mg/kg respectively for 21 days. Group F rats received omeprazole at 1.8 mg/kg for 21 days. Five rats from each group were anaesthetized with ketamine on days 14, 21 and 28. Gastric tissues were excised and fixed in Neutral buffered formalin. This was followed by paraffin wax embedding method and sections stained with haematoxylin and eosin and for immunolocalisation of EGFR and PECAM-1. Data were analysed using descriptive and inferential statistics.

Results: There was a significant difference in the ulcer index in the corpus of control and treated rats throughout the experimental period (p = 0.0001). H&E stained sections showed a gradual restoration of the epithelial lining in the treated groups. Immunohistochemical examination showed that significantly increased (p < 0.05) reactivity for both EGFR and CD31 across the treatment groups.

Conclusion: The efficacy of in attenuating the damaging effects of aspirin on the gastric mucosa was observed as there was a significantly increased reactivity for EGFR and PECAM-1 in the gastric corpus in a dose-dependent manner.

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Source
http://dx.doi.org/10.3889/oamjms.2017.058DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5591583PMC
August 2017
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