Microbiome of Pacific Whiteleg shrimp reveals differential bacterial community composition between Wild, Aquacultured and AHPND/EMS outbreak conditions.

Sci Rep 2017 09 18;7(1):11783. Epub 2017 Sep 18.

Departamento de Microbiología Molecular, Instituto de Biotecnología (IBT), Universidad Nacional Autónoma de México (UNAM), Av. Universidad #2001, Col. Chamilpa, Cuernavaca, Morelos, 62210, Mexico.

Crustaceans form the second largest subphylum on Earth, which includes Litopeneaus vannamei (Pacific whiteleg shrimp), one of the most cultured shrimp worldwide. Despite efforts to study the shrimp microbiota, little is known about it from shrimp obtained from the open sea and the role that aquaculture plays in microbiota remodeling. Here, the microbiota from the hepatopancreas and intestine of wild type (wt) and aquacultured whiteleg shrimp and pond sediment from hatcheries were characterized using sequencing of seven hypervariable regions of the 16S rRNA gene. Cultured shrimp with AHPND/EMS disease symptoms were also included. We found that (i) microbiota and their predicted metagenomic functions were different between wt and cultured shrimp; (ii) independent of the shrimp source, the microbiota of the hepatopancreas and intestine was different; (iii) the microbial diversity between the sediment and intestines of cultured shrimp was similar; and (iv) associated to an early development of AHPND/EMS disease, we found changes in the microbiome and the appearance of disease-specific bacteria. Notably, under cultured conditions, we identified bacterial taxa enriched in healthy shrimp, such as Faecalibacterium prausnitzii and Pantoea agglomerans, and communities enriched in diseased shrimp, such as Aeromonas taiwanensis, Simiduia agarivorans and Photobacterium angustum.

Download full-text PDF

Source
http://www.nature.com/articles/s41598-017-11805-w
Publisher Site
http://dx.doi.org/10.1038/s41598-017-11805-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5603525PMC
September 2017
6 Reads

Publication Analysis

Top Keywords

cultured shrimp
16
shrimp
12
whiteleg shrimp
12
ahpnd/ems disease
8
pacific whiteleg
8
microbiota hepatopancreas
8
hepatopancreas intestine
8
cultured
5
microbiota
5
predicted metagenomic
4
microbiota predicted
4
symptoms included
4
disease symptoms
4
metagenomic functions
4
included microbiota
4
independent shrimp
4
intestine iii
4
iii microbial
4
source microbiota
4
shrimp source
4

Similar Publications