MicroRNA‑106b regulates skeletal muscle insulin sensitivity and glucose homeostasis by targeting mitofusion‑2.

Mol Med Rep 2017 Nov 8;16(5):6858-6863. Epub 2017 Sep 8.

Department of Pediatrics, Jinling Hospital, Nanjing University School of Medicine, Nanjing, Jiangsu 210002, P.R. China.

MicroRNA‑106b (miR‑106b) is reported to be closely associated with skeletal muscle insulin resistance. The present study further investigated the role of miR‑106b in skeletal muscle insulin sensitivity and glucose homeostasis in vivo. Mice were randomly divided into 4 groups and infected with lentivirus expressing miR‑106b (miR‑106b mice), miR‑106b sponge (miR‑106b inhibition mice) or the corresponding empty vectors. Mitofusion‑2 (Mfn2) protein expression levels and glucose transporter (Glut)‑4 protein translocation were significantly reduced in the muscle of miR‑106b mice, whereas they were unaffected in miR‑106b inhibition mice. miR‑106b mice had significantly increased blood glucose levels following 12 h of fasting and impaired glucose tolerance, whereas miR‑106b inhibition mice had no significant alterations in fasting blood glucose levels and glucose tolerance. In vitro, the suppressive effect of miR‑106b on glucose uptake and Glut4 translocation was completely inhibited in C2C12 myotubes infected with Mfn2 plasmids. Following treatment of C2C12 myotubes with Mfn2 small interfering RNA, miR‑106b inhibition consistently increased Mfn2 protein levels and improved glucose uptake and Glut4 translocation. These results indicated that miR‑106b targeted Mfn2 and regulated skeletal muscle insulin sensitivity and glucose tolerance. Therefore, increased miR‑106b expression may be a potential mechanism underlying insulin resistance and type 2 diabetes.

Download full-text PDF

Source
https://www.spandidos-publications.com/10.3892/mmr.2017.7439
Publisher Site
http://dx.doi.org/10.3892/mmr.2017.7439DOI Listing
November 2017
13 Reads

Publication Analysis

Top Keywords

muscle insulin
16
skeletal muscle
16
mir‑106b inhibition
16
mir‑106b
14
inhibition mice
12
sensitivity glucose
12
mir‑106b mice
12
glucose tolerance
12
insulin sensitivity
12
glucose
10
uptake glut4
8
glut4 translocation
8
levels glucose
8
c2c12 myotubes
8
glucose uptake
8
mfn2 protein
8
glucose levels
8
blood glucose
8
glucose homeostasis
8
mice mir‑106b
8

Similar Publications