BMC Biotechnol 2017 Sep 9;17(1):71. Epub 2017 Sep 9.
Laboratory of Genomics and Expression, Department of Genetics and Evolution, Institute of Biology, UNICAMP, Campinas, São Paulo, 13083-970, Brazil.
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J Biosci Bioeng 2016 Jun 30;121(6):685-691. Epub 2015 Nov 30.
College of Architecture and Environment, Sichuan University, No. 24, South Section 1, First Ring Road, Chengdu 610065, China.
Saccharomyces cerevisiae strains with xylose isomerase (XI) pathway were constructed using a flocculating industrial strain (YC-8) as the host. Both strains expressing wild-type xylA (coding XI) from the fungus Orpinomyces sp. and the bacterium Prevotella ruminicola, respectively, showed better growth ability and fermentation capacity when using xylose as the sole sugar than most of the reported strains expressing XI. Read More
Microb Cell Fact 2015 May 17;14:70. Epub 2015 May 17.
School of Life Sciences and Chemical Technology, Ngee Ann Polytechnic, Singapore, Singapore.
Background: Xylose isomerase (XI) catalyzes the conversion of xylose to xylulose, which is the key step for anaerobic ethanolic fermentation of xylose. Very few bacterial XIs can function actively in Saccharomyces cerevisiae. Here, we illustrate a group of XIs that would function for xylose fermentation in S. Read More
Adv Biochem Eng Biotechnol 2007 ;108:179-204
Department of Biotechnology, Delft University of Technology, Julianalaan 67, 2628 BC, Delft, The Netherlands.
Metabolic engineering of Saccharomyces cerevisiae for ethanol production from D-xylose, an abundant sugar in plant biomass hydrolysates, has been pursued vigorously for the past 15 years. Whereas wild-type S. cerevisiae cannot ferment D-xylose, the keto-isomer D-xylulose can be metabolised slowly. Read More
Biotechnol Bioeng 2016 10 6;113(10):2149-55. Epub 2016 Apr 6.
Carl R. Woese Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, Illinois.
Rapid advances in the capabilities of reading and writing DNA along with increasing understanding of microbial metabolism at the systems-level have paved an incredible path for metabolic engineering. Despite these advances, post-translational tools facilitating functional expression of heterologous enzymes in model hosts have not been developed well. Some bacterial enzymes, such as Escherichia coli xylose isomerase (XI) and arabinose isomerase (AI) which are essential for utilizing cellulosic sugars, cannot be functionally expressed in Saccharomyces cerevisiae. Read More