Development of high-throughput and high sensitivity capillary gel electrophoresis platform method for Western, Eastern, and Venezuelan equine encephalitis (WEVEE) virus like particles (VLPs) purity determination and characterization.

Authors:
Diane L Wycuff
Diane L Wycuff
Baylor University
United States
Richard M Schwartz
Richard M Schwartz
Cleveland Clinic Foundation
United States
Jonathan W Cooper
Jonathan W Cooper
University of Maryland
United States

Electrophoresis 2017 10 20;38(20):2610-2621. Epub 2017 Sep 20.

Vaccine Production Program, VRC, NIAID, NIH, Gaithersburg, MD, USA.

In this paper, we describe development of a high-throughput, highly sensitive method based on Lab Chip CGE-SDS platform for purity determination and characterization of virus-like particle (VLP) vaccines. A capillary gel electrophoresis approach requiring about 41 s per sample for analysis and demonstrating sensitivity to protein initial concentrations as low as 20 μg/mL, this method has been used previously to evaluate monoclonal antibodies, but this application for lot release assay of VLPs using this platform is unique. The method was qualified and shown to be accurate for the quantitation of VLP purity. Assay repeatability was confirmed to be less than 2% relative standard deviation of the mean (% RSD) with interday precision less than 2% RSD. The assay can evaluate purified VLPs in a concentration range of 20-249 μg/mL for VEE and 20-250 μg/mL for EEE and WEE VLPs.

Download full-text PDF

Source
http://dx.doi.org/10.1002/elps.201700217DOI Listing

Still can't find the full text of the article?

We can help you send a request to the authors directly.
October 2017
52 Reads

Publication Analysis

Top Keywords

capillary gel
8
gel electrophoresis
8
purity determination
8
determination characterization
8
development high-throughput
8
antibodies application
4
monoclonal antibodies
4
method evaluate
4
20 μg/ml method
4
evaluate monoclonal
4
application lot
4
vlps platform
4
release assay
4
lot release
4
low 20 μg/ml
4
platform unique
4
assay vlps
4
initial concentrations
4
vaccines capillary
4
electrophoresis approach
4

Similar Publications