Effects of the Hydroalcoholic Extract of on Arginase I Activity and Expression in the Retina of Streptozotocin-Induced Diabetic Rats.

Int J Endocrinol Metab 2017 Apr 13;15(2):e42161. Epub 2017 Feb 13.

Hyperlipidemia Research Center, Department of Clinical Biochemistry, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IR Iran.

Background: Emerging evidence suggests that an increased arginase activity is involved in vascular dysfunction in experimental animals. Roscoe, commonly known as ginger, has been widely used in the traditional medicine for treatment of diabetes.

Objectives: This study aimed at investigating the effects of the hydroalcoholic extract of on arginase I activity and expression in the retina of streptozotocin (STZ)-induced diabetic rats.

Methods: In this experimental study, 16 male Wistar rats weighing 200 - 250 g were assessed. Diabetes was induced via a single intraperitoneal injection of STZ (60 mg/kg body weight). The rats were randomly allocated into four experimental groups. Untreated healthy and diabetic controls received 1.5 mL/kg distilled water. Treated diabetic rats received 200, and 400 mg/kg of the extract dissolved in distilled water (1.5 mL/kg). Body weight, blood glucose and insulin concentration were measured by standard methods. The arginase I activity and expression were determined by spectrophotometric and western blot analysis, respectively.

Results: Our results showed that blood glucose concentration was significantly decreased in diabetic rats treated with the extract compared to untreated diabetic controls (P < 0.01). Treatment with 400 mg/kg of the extract reduced arginase I activity and expression (P < 0.05). A significant elevation in body weight was observed in diabetic rats treated with the extract. Serum insulin was significantly increased in diabetic rats treated with 400 mg/kg of the extract compared to diabetic controls (P < 0.05).

Conclusions: Our results suggest that the hydroalcoholic extract may potentially be a promising therapeutic option for treating diabetes-induced vascular disorders, possibly through reducing arginase I activity and expression in the retina.

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Source
http://dx.doi.org/10.5812/ijem.42161DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5555732PMC
April 2017
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