The draft genome of the hyperthermophilic archaeon strain hulk, an iron and nitrate reducer, reveals the capacity for sulfate reduction.

Authors:
Lucas M Demey
Lucas M Demey
Michigan State University
East Lansing | United States
Caitlin R Miller
Caitlin R Miller
Michigan State University
East Lansing | United States
Michael P Manzella
Michael P Manzella
Michigan State University
United States
Rachel R Spurbeck
Rachel R Spurbeck
University of Michigan Medical School
United States
Sukhinder K Sandhu
Sukhinder K Sandhu
Swift Biosciences
Gemma Reguera
Gemma Reguera
University of Massachusetts
United States
Kazem Kashefi
Kazem Kashefi
University of Massachusetts
United States

Stand Genomic Sci 2017 15;12:47. Epub 2017 Aug 15.

Department of Microbiology and Molecular Genetics, Michigan State University, East Lansing, MI USA.

strain Hulk is a newly sequenced strain isolated from chimney samples collected from the Hulk sulfide mound on the main Endeavour Segment of the Juan de Fuca Ridge (47.9501 latitude, -129.0970 longitude, depth 2200 m) in the Northeast Pacific Ocean. The draft genome of strain Hulk shared 99.77% similarity with the complete genome of the type strain Su06, which shares with strain Hulk the ability to reduce iron and nitrate for respiration. The annotation of the genome of strain Hulk identified genes for the reduction of several sulfur-containing electron acceptors, an unsuspected respiratory capability in this species that was experimentally confirmed for strain Hulk. This makes strain Hulk the first hyperthermophilic archaeon known to gain energy for growth by reduction of iron, nitrate, and sulfur-containing electron acceptors. Here we present the most notable features of the genome of strain Hulk and identify genes encoding proteins critical to its respiratory versatility at high temperatures. The description presented here corresponds to a draft genome sequence containing 2,042,801 bp in 9 contigs, 2019 protein-coding genes, 53 RNA genes, and 1365 hypothetical genes.

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Source
http://dx.doi.org/10.1186/s40793-017-0260-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5556600PMC
August 2017
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References

(Supplied by CrossRef)
Article in Extremophiles
E Blöchl et al.
Extremophiles 1997
Article in System Appl Microbiol
U Pley et al.
System Appl Microbiol 1991
Article in J Bacteriol
W Zillig et al.
J Bacteriol 1990
Article in Geochim Cosmochim Acta
TM McCollom et al.
Geochim Cosmochim Acta 2001
Article in Am J Analyt Chem
C Konn et al.
Am J Analyt Chem 2015
Article in Environ Microbiol
JM Tor et al.
Environ Microbiol 2003
Article in Geochim Cosmochim Acta
SQ Lang et al.
Geochim Cosmochim Acta 2010
Article in J Gen Appl Microbiol
W Zhang et al.
J Gen Appl Microbiol 2014
Article in Appl Environ Microbiol
MP Manzella et al.
Appl Environ Microbiol 2013
Article in Geomicrobiol J
SN Gavrilov et al.
Geomicrobiol J 2012

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