RGD peptide and graphene oxide co-functionalized PLGA nanofiber scaffolds for vascular tissue engineering.

Regen Biomater 2017 Jun 7;4(3):159-166. Epub 2017 Feb 7.

Department of Cogno-Mechatronics Engineering.

In recent years, much research has been suggested and examined for the development of tissue engineering scaffolds to promote cellular behaviors. In our study, RGD peptide and graphene oxide (GO) co-functionalized poly(lactide--glycolide, PLGA) (RGD-GO-PLGA) nanofiber mats were fabricated via electrospinning, and their physicochemical and thermal properties were characterized to explore their potential as biofunctional scaffolds for vascular tissue engineering. Scanning electron microscopy images revealed that the RGD-GO-PLGA nanofiber mats were readily fabricated and composed of random-oriented electrospun nanofibers with average diameter of 558 nm. The successful co-functionalization of RGD peptide and GO into the PLGA nanofibers was confirmed by Fourier-transform infrared spectroscopic analysis. Moreover, the surface hydrophilicity of the nanofiber mats was markedly increased by co-functionalizing with RGD peptide and GO. It was found that the mats were thermally stable under the cell culture condition. Furthermore, the initial attachment and proliferation of primarily cultured vascular smooth muscle cells (VSMCs) on the RGD-GO-PLGA nanofiber mats were evaluated. It was revealed that the RGD-GO-PLGA nanofiber mats can effectively promote the growth of VSMCs. In conclusion, our findings suggest that the RGD-GO-PLGA nanofiber mats can be promising candidates for tissue engineering scaffolds effective for the regeneration of vascular smooth muscle.

Download full-text PDF

Source
http://dx.doi.org/10.1093/rb/rbx001DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5516678PMC
June 2017
12 Reads

Publication Analysis

Top Keywords

nanofiber mats
24
rgd-go-plga nanofiber
20
rgd peptide
16
tissue engineering
16
engineering scaffolds
8
scaffolds vascular
8
vascular tissue
8
mats fabricated
8
revealed rgd-go-plga
8
vascular smooth
8
smooth muscle
8
oxide co-functionalized
8
peptide graphene
8
graphene oxide
8
mats
7
nanofiber
7
rgd-go-plga
5
nanofibers average
4
cells vsmcs
4
diameter 558 nm
4

Similar Publications