Pediatric dilated cardiomyopathy hearts display a unique gene expression profile.

JCI Insight 2017 Jul 20;2(14). Epub 2017 Jul 20.

Division of Cardiology, University of Colorado School of Medicine, Aurora, Colorado, USA.

Our previous work showed myocellular differences in pediatric and adult dilated cardiomyopathy (DCM). However, a thorough characterization of the molecular pathways involved in pediatric DCM does not exist, limiting the development of age-specific therapies. To characterize this patient population, we investigated the transcriptome profile of pediatric patients. RNA-Seq from 7 DCM and 7 nonfailing (NF) explanted age-matched pediatric left ventricles (LV) was performed. Changes in gene expression were confirmed by real-time PCR (RT-PCR) in 36 DCM and 21 NF pediatric hearts and in 20 DCM and 10 NF adult hearts. The degree of myocyte hypertrophy was investigated in 4 DCM and 7 NF pediatric hearts and in 4 DCM and 9 NF adult hearts. Changes in gene expression in response to pluripotency-inducing factors were investigated in neonatal rat ventricular myocytes (NRVMs). Transcriptome analysis identified a gene expression profile in children compared with adults with DCM. Additionally, myocyte hypertrophy was not observed in pediatric hearts but was present in adult hearts. Furthermore, treatment of NRVMs with pluripotency-inducing factors recapitulated changes in gene expression observed in the pediatric DCM heart. Pediatric DCM is characterized by unique changes in gene expression that suggest maintenance of an undifferentiated state.

Download full-text PDF

Source
http://dx.doi.org/10.1172/jci.insight.94249DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5518568PMC
July 2017
52 Reads

Publication Analysis

Top Keywords

gene expression
24
changes gene
16
adult hearts
12
pediatric dcm
12
pediatric hearts
12
pediatric
10
dcm
10
dilated cardiomyopathy
8
dcm adult
8
expression profile
8
pluripotency-inducing factors
8
dcm pediatric
8
hearts dcm
8
myocyte hypertrophy
8
observed pediatric
8
hearts
7
expression
6
gene
6
expression response
4
response pluripotency-inducing
4

Altmetric Statistics

References

(Supplied by CrossRef)

Similar Publications