Validation of OncoPanel: A Targeted Next-Generation Sequencing Assay for the Detection of Somatic Variants in Cancer.

Arch Pathol Lab Med 2017 Jun 3;141(6):751-758. Epub 2017 Mar 3.

From the Center for Advanced Molecular Diagnostics, Brigham and Women's Hospital (Drs Garcia, Minkovsky, Jia, Ligon, Sholl, Kuo, MacConaill, Lindeman, and Dong, Messrs Ducar and Gong, and Ms. Shivdasani), and the Center for Cancer Genome Discovery, Dana Farber Cancer Institute (Mr Ducar and Dr MacConaill), Harvard Medical School, Boston, Massachusetts.

Context: - The analysis of somatic mutations across multiple genes in cancer specimens may be used to aid clinical decision making. The analytical validation of targeted next-generation sequencing panels is important to assess accuracy and limitations.

Objective: - To report the development and validation of OncoPanel, a custom targeted next-generation sequencing assay for cancer.

Design: - OncoPanel was designed for the detection of single-nucleotide variants, insertions and deletions, copy number alterations, and structural variants across 282 genes with evidence as drivers of cancer biology. We implemented a validation strategy using formalin-fixed, paraffin-embedded, fresh or frozen samples compared with results obtained by clinically validated orthogonal technologies.

Results: - OncoPanel achieved 98% sensitivity and 100% specificity for the detection of single-nucleotide variants, and 84% sensitivity and 100% specificity for the detection of insertions and deletions compared with single-gene assays and mass spectrometry-based genotyping. Copy number detection achieved 86% sensitivity and 98% specificity compared with array comparative genomic hybridization. The sensitivity of structural variant detection was 74% compared with karyotype, fluorescence in situ hybridization, and polymerase chain reaction. Sensitivity was affected by inconsistency in the detection of FLT3 and NPM1 alterations and IGH rearrangements due to design limitations. Limit of detection studies demonstrated 98.4% concordance across triplicate runs for variants with allele fraction greater than 0.1 and at least 50× coverage.

Conclusions: - The analytical validation of OncoPanel demonstrates the ability of targeted next-generation sequencing to detect multiple types of genetic alterations across a panel of genes implicated in cancer biology.

Download full-text PDF

Source
http://dx.doi.org/10.5858/arpa.2016-0527-OADOI Listing
June 2017
110 Reads

Publication Analysis

Top Keywords

next-generation sequencing
16
targeted next-generation
16
validation oncopanel
12
detection single-nucleotide
8
insertions deletions
8
cancer biology
8
specificity detection
8
analytical validation
8
100% specificity
8
sensitivity 100%
8
detection
8
sequencing assay
8
copy number
8
single-nucleotide variants
8
variants
5
validation
5
sensitivity
5
spectrometry-based genotyping
4
number detection
4
detection achieved
4

Similar Publications