BMC Biophys 2017 22;10. Epub 2017 May 22.
Laboratory of Biotechnology and Molecular Biology, MASIRA Institute, School of Health, University of Santander, UDES, Bucaramanga, Colombia.
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J Econ Entomol 2004 Dec;97(6):1805-13
AgResearch Ltd., Wallaceville Animal Research Centre, Ward Street, Upper Hutt, New Zealand.
Bacillus thuringiensis that produce Cry1Ba are toxic to Lucilia cuprina Wiedemann blow fly maggots in vivo, and when applied in quantity to sheep fleece, provide up to 6 wk protection against flystrike in the field. These strains also are toxic to Epiphyas postvittana (Walker) light brown apple moth caterpillars. B. Read More
Curr Microbiol 1999 Jul;39(1):14-20
Graduate School of Bio-Applications and Systems Engineering, Tokyo University of Agriculture and Technology, Koganei, Tokyo 184-8588, Japan.
We investigated the binding proteins for three Cry toxins, Cry1Aa, Cry1Ac, and the phylogenetically distant Cry9Da, in the midgut cell membrane of the silkworm. In a ligand blot experiment, Cry1Ac and Cry9Da bound to the same 120-kDa aminopeptidase N (APN) as Cry1Aa. A competition experiment with the ligand blot indicated that the three toxins share the same binding site on several proteins. Read More
Wei Sheng Wu Xue Bao 2008 Sep;48(9):1250-5
Key Laboratory of Molecular Microbiology of Hunan Province, College of Life Science, Hunan Normal University, Changsha 410081, China.
Objective: To localize and identify the insecticidal crystal protein genes (cry genes) in high-toxic Bacillus thuringiensis strain 4.0718.
Methods: The genomic DNA of B. Read More
Appl Environ Microbiol 1996 Apr;62(4):1369-77
Institute of Biochemistry, National Yang Ming University, Taipei, Taiwan, Republic of China.
Two pairs of universal oligonucleotide primers were designed to probe the most conserved regions of all known cryI-type gene sequences so that the amplified PCR fragments of the DNA template from Bacillus thuringiensis strains may contain all possible cryI-type gene sequences. The restriction fragment length polymorphism (RFLP) patterns of the PCR-amplified fragments revealed that 14 distinct cry-type genes have been identified from 20 B. thuringiensis strains. Read More