Thermoresponsive pegylated bubble liposome nanovectors for efficient siRNA delivery via endosomal escape.

Authors:
Kholod Alamoudi
Kholod Alamoudi
Smart Hybrid Materials Laboratory
Patricia Martins
Patricia Martins
Institute of Physical Activity and Sports Sciences
Dr. Jonas G Croissant, PhD
Dr. Jonas G Croissant, PhD
University of New Mexico, Chemical & Biological Engineering
Research Assistant Professor
Chemistry, Materials Science
Albuquerque, New Mexico | United States
Sachin Patil
Sachin Patil
Saint Barnabas Medical Center
Livingston | United States
Haneen Omar
Haneen Omar
Smart Hybrid Materials Laboratory
Niveen M Khashab
Niveen M Khashab
Smart Hybrid Materials Laboratory
United States

Nanomedicine (Lond) 2017 May 19. Epub 2017 May 19.

Smart Hybrid Materials Laboratory, Advanced Membranes & Porous Materials Center, King Abdullah University of Science & Technology, Thuwal, Saudi Arabia.

Aim: Improving the delivery of siRNA into cancer cells via bubble liposomes. Designing a thermoresponsive pegylated liposome through the introduction of ammonium bicarbonate salt into liposomes so as to control their endosomal escape for gene therapy.

Methods: A sub-200 nm nanovector was fully characterized and examined for cellular uptake, cytotoxicity, endosomal escape and gene silencing.

Results: The siRNA-liposomes were internalized into cancer cells within 5 min and then released siRNAs in the cytosol prior to lysosomal degradation upon external temperature elevation. This was confirmed by confocal bioimaging and gene silencing reaching up to 90% and further demonstrated by the protein inhibition of both target genes.

Conclusion: The thermoresponsiveness of ammonium bicarbonate containing liposomes enabled the rapid endosomal escape of the particles and resulted in an efficient gene silencing.

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http://dx.doi.org/10.2217/nnm-2017-0021DOI Listing

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May 2017
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