Stromal SPOCK1 supports invasive pancreatic cancer growth.

Mol Oncol 2017 08 5;11(8):1050-1064. Epub 2017 Jun 5.

Laboratory for Experimental Oncology and Radiobiology, Center for Experimental and Molecular Medicine, Academic Medical Center and Cancer Center Amsterdam, The Netherlands.

Pancreatic ductal adenocarcinoma (PDAC) is marked by an abundant stromal deposition. This stroma is suspected to harbor both tumor-promoting and tumor-suppressing properties. This is underscored by the disappointing results of stroma targeting in clinical studies. Given the complexity of tumor-stroma interaction in PDAC, there is a need to identify the stromal proteins that are predominantly tumor-promoting. One possible candidate is SPOCK1 that we previously identified in a screening effort in PDAC. We extensively mined PDAC gene expression datasets, and used species-specific transcript analysis in mixed-species models for PDAC to study the patterns and driver mechanisms of SPOCK1 expression in PDAC. Advanced organotypic coculture models with primary patient-derived tumor cells were used to further characterize the function of this protein. We found SPOCK1 expression to be predominantly stromal. Expression of SPOCK1 was associated with poor disease outcome. Coculture and ligand stimulation experiments revealed that SPOCK1 is expressed in response to tumor cell-derived transforming growth factor-beta. Functional assessment in cocultures demonstrated that SPOCK1 strongly affects the composition of the extracellular collagen matrix and by doing so, enables invasive tumor cell growth in PDAC. By defining the expression pattern and functional properties of SPOCK1 in pancreatic cancer, we have identified a stromal mediator of extracellular matrix remodeling that indirectly affects the aggressive behavior of PDAC cells. The recognition that stromal proteins actively contribute to the protumorigenic remodeling of the tumor microenvironment should aid the design of future clinical studies to target specific stromal targets.

Download full-text PDF

Source
http://dx.doi.org/10.1002/1878-0261.12073DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5537700PMC
August 2017
39 Reads

Publication Analysis

Top Keywords

spock1 expression
8
pancreatic cancer
8
stromal proteins
8
pdac
8
clinical studies
8
stromal
7
spock1
7
expression
5
revealed spock1
4
experiments revealed
4
ligand stimulation
4
poor disease
4
disease outcome
4
outcome coculture
4
coculture ligand
4
stimulation experiments
4
spock1 expressed
4
growth factor-beta
4
factor-beta functional
4
functional assessment
4

Similar Publications