The first fluorogenic sensor for sphingosine-1-phosphate lyase activity in intact cells.

Chem Commun (Camb) 2017 May;53(39):5441-5444

Spanish National Research Council (CSIC), Institute of Advanced Chemistry of Catalonia (IQAC-CSIC), Research Unit on Bioactive Molecules (RUBAM), Department of Biomedicinal Chemistry, Jordi Girona 18 26, 08034 Barcelona, Spain.

New fluorogenic sensors with suitable kinetic parameters and sensitivity have been developed for the determination of sphingosine-1-phosphate lyase activity in cell lysates. The probe RBM148 can be efficiently loaded into cationic liposomes and used to determine S1PL activity in intact cells.

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http://dx.doi.org/10.1039/c7cc01659jDOI Listing
May 2017
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