Enhanced Probiotic Potential of When Delivered as a Biofilm on Dextranomer Microspheres That Contain Beneficial Cargo.

Front Microbiol 2017 27;8:489. Epub 2017 Mar 27.

Center for Microbial Pathogenesis, The Research Institute at Nationwide Children's Hospital Columbus, OH, USA.

As with all orally consumed probiotics, the Gram-positive bacterium encounters numerous challenges as it transits through the gastrointestinal tract of the host, including low pH, effectors of the host immune system, as well as competition with commensal and pathogenic bacteria, all of which can greatly reduce the availability of live bacteria for therapeutic purposes. Recently we showed that , when adhered in the form of a biofilm to a semi-permeable biocompatible dextranomer microsphere, reduces the incidence of necrotizing enterocolitis by 50% in a well-defined animal model following delivery of a single prophylactic dose. Herein, using the same semi-permeable microspheres, we showed that providing compounds beneficial to as diffusible cargo within the microsphere lumen resulted in further advantageous effects including glucosyltransferase-dependent bacterial adherence to the microsphere surface, resistance of bound bacteria against acidic conditions, enhanced adherence of to human intestinal epithelial cells , and facilitated production of the antimicrobial compound reuterin and the anti-inflammatory molecule histamine. These data support continued development of this novel probiotic formulation as an adaptable and effective means for targeted delivery of cargo beneficial to the probiotic bacterium.

Download full-text PDF

Source
http://dx.doi.org/10.3389/fmicb.2017.00489DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5366311PMC
March 2017
28 Reads

Publication Analysis

Top Keywords

incidence necrotizing
4
necrotizing enterocolitis
4
compound reuterin
4
reduces incidence
4
reuterin anti-inflammatory
4
microsphere reduces
4
antimicrobial compound
4
enterocolitis 50%
4
model delivery
4
delivery single
4
animal model
4
production antimicrobial
4
50% well-defined
4
well-defined animal
4
dextranomer microsphere
4
biocompatible dextranomer
4
live bacteria
4
acidic conditions
4
availability live
4
histamine data
4

References

(Supplied by CrossRef)

Araya et al.
2006

Similar Publications