Anticancer Res 2017 04;37(4):1603-1608
Graduate School of Humanity-Oriented Science and Engineering, Kindai University, Fukuoka, Japan.
Background/aim: The transcription factor Y-box-binding protein 1 (YB1) is overexpressed in many types of human cancers. YB1 regulates the G phase of the cell cycle by controlling transcription of G regulators. Here, we report that YB1 is also involved in regulating G/M phase.
Materials And Methods: YB1-depleted TKO cells were subjected to quantitative reverse transcription-polymerase chain reaction and cell-cycle analysis. RNA immunoprecipitation (RIP)-chip assay was performed using anti-YB1 antibodies. Precipitated RNAs were subjected to microarray analysis.
Results: Silencing YB1 inhibited the proliferation of TKO cells, which lost the machinery required for G phase arrest. Cell-cycle analysis showed that silencing YB1 caused G/M phase cell-cycle arrest. RIP-chip assay showed that YB1 associated with mRNA of multiple cell-cycle-related genes, including G/M phase regulators.
Conclusion: YB1 positively regulates not only the G phase but also G/M phase by regulating multiple cell-cycle-related genes.