BMC Biotechnol 2017 03 27;17(1):34. Epub 2017 Mar 27.
Animal Biotechnology, Graduate School of Future Convergence Technology, Institute of Genetic Engineering, Hankyong National University, Ansung, 17579, Korea.
Download full-text PDF
Physiol Genomics 2008 Mar 18;33(1):65-77. Epub 2007 Dec 18.
Department of Animal Sciences, University of Illinois at Urbana-Champaign, Urbana, Illinois, USA.
Transcription profiling of placentomes derived from somatic cell nuclear transfer (SCNT, n = 20), in vitro fertilization (IVF, n = 9), and artificial insemination (AI, n = 9) at or near term development was performed to better understand why SCNT and IVF often result in placental defects, hydrops, and large offspring syndrome (LOS). Multivariate analysis of variance was used to distinguish the effects of SCNT, IVF, and AI on gene expression, taking into account the effects of parturition (term or preterm), sex of fetus, breed of dam, breed of fetus, and pathological finding in the offspring (hydrops, normal, or other abnormalities). Differential expression of 20 physiologically important genes was confirmed with quantitative PCR. Read More
Cell Reprogram 2010 Apr;12(2):213-22
College of Life Science, Northeast Agricultural University , Harbin, People's Republic of China.
Unlike embryos derived from fertilization, most cloned embryos die during postimplantation development, and those that survive to term are frequently defective. Many of the observed defects involve placenta. Abnormal placentation has been described in several cloned species. Read More
Physiol Genomics 2013 Jan 23;45(1):28-46. Epub 2012 Oct 23.
Institute of Animal Science, Animal Breeding and Husbandry Group, University of Bonn, Bonn, Germany.
In the present study, we used the global transcriptome profile approach to identify dysregulated genes, molecular pathways, and molecular functional alterations in bovine placentas derived from somatic cell nuclear transfer (SCNT) and in vitro embryo production (IVP) pregnancies compared with their artificial insemination (AI) counterparts at day 50 of gestation. For this, day 7 blastocysts derived from AI, IVP, or SCNT were transferred to oestrus-synchronized cows. The pregnant animals were slaughtered at day 50 of gestation, and the placentas were then recovered and used for transcriptome analysis using Affymetrix GeneChip bovine genome array. Read More
PLoS One 2013 16;8(10):e76422. Epub 2013 Oct 16.
RIKEN BioResouce Center, Tsukuba, Ibaraki, Japan.
The great majority of embryos generated by somatic cell nuclear transfer (SCNT) display defined abnormal phenotypes after implantation, such as an increased likelihood of death and abnormal placentation. To gain better insight into the underlying mechanisms, we analyzed genome-wide gene expression profiles of day 6.5 postimplantation mouse embryos cloned from three different cell types (cumulus cells, neonatal Sertoli cells and fibroblasts). Read More