Development of microsatellite markers using Illumina MiSeq sequencing to characterize (Ephedraceae).

Appl Plant Sci 2017 Mar 10;5(3). Epub 2017 Mar 10.

School of Life Sciences, Fudan University, Shanghai 200438, People's Republic of China; Science Faculty, Tibet University, Lhasa 850000, Tibet Autonomous Region, People's Republic of China.

Premise Of The Study: (Ephedraceae), occurring in the Himalayan ranges, is an important plant species used in Tibetan medicine. Due to the lack of molecular markers to characterize genetic diversity, knowledge for conservation and uses of resources is limited; we therefore developed microsatellite markers for use in this species.

Methods And Results: Using Illumina MiSeq sequencing technology, we developed 29 polymorphic microsatellite loci suitable for , of which 15 loci also showed polymorphisms in two related species, and . The average number of effective alleles per locus ranged from two to six. The observed and expected heterozygosity ranged from 0.23 to 0.83 and 0.44 to 0.86, respectively, in populations.

Conclusions: The developed 29 microsatellite markers are effective for the study of genetic structure and genetic diversity of , and 15 of these markers are suitable for related species.

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Source
http://doi.wiley.com/10.3732/apps.1600104
Publisher Site
http://dx.doi.org/10.3732/apps.1600104DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5357120PMC
March 2017
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