Real-Time PCR Identification of Six Malassezia Species.

Authors:
Amin Ilahi
Amin Ilahi
Laboratoire de biologie moléculaire parasitaire et fongique
Ines Hadrich
Ines Hadrich
School of Medicine
Sourour Neji
Sourour Neji
Sfax School of Medicine
Houaida Trabelsi
Houaida Trabelsi
Fungal and parasitic molecular biology laboratory- Sfax
Fattouma Makni
Fattouma Makni
School of Medicine
Ali Ayadi
Ali Ayadi
School of Medicine

Curr Microbiol 2017 Jun 22;74(6):671-677. Epub 2017 Mar 22.

Fungal and Parasitic Molecular Biology Laboratory, Faculty of Medicine, University of Sfax, Rue Magida Boulila, Sfax, 3029, Tunisia.

Lipophilic yeast Malassezia species is widely found on the skin surface of humans and other animals. This fungus can cause pityriasis versicolor, Malassezia folliculitis, and seborrheic dermatitis. Still now, there is a problem with species identification of Malassezia with conventional methods. We developed a real-time polymerase chain reaction (PCR) assay with multiple hybridization probes for detecting M. globosa, M. furfur, M. restricta, M. sympodialis, M. slooffiae, and M. pachydermatis. The amplification curves and specific melting peaks of the probes hybridized with real-time PCR product were used for species identifications. The assay was further evaluated on 120 samples which were performed by swabbing from 60 domestic animals (23 goats, 10 dogs, 15 cows, 3 cats, 8 rabbits, and 1 donkey) and in 70 human samples (28 patients with pityriasis versicolor, 17 breeders, and 25 control group). Fifteen M. pachydermatis were identified from animals. From human, 61 isolates were identified as M. globosa (28), M. furfur (15), M. restricta (6), M. sympodialis (8), M. slooffiae (2), and M. pachydermatis (2). Eight cases of co-detection from 6 patients and 2 breeders were revealed. Our findings show that the assay was highly effective in identifying Malassezia species. The application of multiplex real-time PCR provides a sensitive and rapid identification system for Malassezia species, which may be applied in further epidemiological surveys from clinical samples.

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00284-017-1237-7DOI Listing

Still can't find the full text of the article?

We can help you send a request to the authors directly.
June 2017
16 Reads

Publication Analysis

Top Keywords

malassezia species
16
real-time pcr
12
sympodialis slooffiae
8
restricta sympodialis
8
pityriasis versicolor
8
globosa furfur
8
furfur restricta
8
identification malassezia
8
slooffiae pachydermatis
8
species
6
malassezia
6
animals goats
4
goats dogs
4
performed swabbing
4
dogs cows
4
swabbing domestic
4
domestic animals
4
rabbits donkey
4
human samples
4
samples patients
4

Similar Publications