BMC Biotechnol 2017 03 14;17(1):29. Epub 2017 Mar 14.
Hainan Key Laboratory for Sustainable Utilization of Tropical Bioresources, College of Agriculture, Hainan University, Haikou, Hainan Province, 570228, China.
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BMC Biotechnol 2016 06 24;16(1):53. Epub 2016 Jun 24.
Ciencias Agrogenómicas, Escuela Nacional de Estudios Superiores, Universidad Nacional Autónoma de México (UNAM), León, C.P.37684, Guanajuato, Mexico.
Background: Phaseolus vulgaris is one of the most extensively studied model legumes in the world. The P. vulgaris genome sequence is available; therefore, the need for an efficient and rapid transformation system is more imperative than ever. Read More
PLoS One 2012 13;7(9):e44908. Epub 2012 Sep 13.
Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee, United States of America.
Background: Populus is a model woody plant and a promising feedstock for lignocellulosic biofuel production. However, its lengthy life cycle impedes rapid characterization of gene function.
Methodology/principal Findings: We optimized a Populus leaf mesophyll protoplast isolation protocol and established a Populus protoplast transient expression system. Read More
PLoS One 2017 21;12(3):e0172475. Epub 2017 Mar 21.
Co-Innovation Center for the Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing, China.
Liriodendron is a genus of the magnolia family comprised of two flowering tree species that produce hardwoods of great ecological and economic value. However, only a limited amount of genetic research has been performed on the Liriodendron genus partly because transient or stable transgenic trees have been difficult to produce. In general, transient expression systems are indispensable for rapid, high-throughput screening and systematic characterization of gene functions at a low cost; therefore, development of such a system for Liriodendron would provide a necessary step forward for research on Magnoliaceae and other woody trees. Read More
Sheng Wu Gong Cheng Xue Bao 2013 Feb;29(2):224-34
Biotechnology Research Institute, Chinese Academy ofAgricultural Sciences, Beijing 100081, China.
Maize (Zea mays L.), wheat (Triticum aestivum L.) and rice (Oryza sativa L. Read More