The role of miR-17-92 cluster in the expression of tumor suppressor genes in unrestricted somatic stem cells.

Biologicals 2017 Mar 17;46:143-147. Epub 2017 Feb 17.

Department of Molecular Medicine, School of Medicine, Qazvin University of Medical Science, Qazvin, Iran. Electronic address:

The miR-17-92 cluster consisted of seven miRNAs (mir-17-5p, -17-3p, -18a, -19a, -20a, -19b-1, and -92a-1). Previous studies have shown this cluster has been over-expressed in several cancers. The aim of this study was to evaluate the over-expression impacts of miR-17-92 on stem cells. In the current work, the effect of miR-17-92 cluster which was cloned in Lentiviral vector has been investigated on unrestricted somatic stem cells (USSCs). Tumor suppressor genes (p53, p15, RBL1, SMAD2, SMAD4, and MAPK-1) expression, especially p53, was considerably reduced. These data show the potential of miR-17-92 for oncogenesis regulation in stem cells. In conclusion, the role of miR-17-92 in USSCs may provide a better understanding of its function in tumorigenesis and for the possible use in cell therapy of the anti-mir-17-92 cluster.

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http://dx.doi.org/10.1016/j.biologicals.2017.02.006DOI Listing
March 2017
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