Data supporting the effects of lysozyme on mRNA and protein expression in a colonic epithelial scratch wound model.

Authors:
Sarah K Abey
Sarah K Abey
National Institutes of Health
United States
Yuana Yuana
Yuana Yuana
Academic Medical Centre of the University of Amsterdam
Netherlands
Dr. Paule V Joseph, PhD, RN, MS, FNP-BC, CTN-B
Dr. Paule V Joseph, PhD, RN, MS, FNP-BC, CTN-B
National Institute of Nursing Research
Tenure-Track Investigator (Clinical)
N/A
Bethesda , Maryland | United States
Natnael D Kenea
Natnael D Kenea
National Institutes of Health
Nicolaas H Fourie
Nicolaas H Fourie
University of Cape Town
South Africa
Leeanne B Sherwin
Leeanne B Sherwin
National Institutes of Health
United States
Gregory E Gonye
Gregory E Gonye
Thomas Jefferson University
United States
Paul A Smyser
Paul A Smyser
National Institute of Nursing Research

Data Brief 2017 Apr 29;11:15-18. Epub 2016 Dec 29.

Digestive Disorders Unit, Division of Intramural Research, National Institutes of Health, Department of Health and Human Services, Bethesda, MD, USA.

Colonic epithelial health is implicated in a host of gastrointestinal (GI) diseases and disorders. Lysozyme is suspected to play a role in the ability of the epithelium to recover from injury (Abey et al., in press; Gallo, 2012; Rubio, 2014) [1], [2], [3]. Disrupted repair mechanisms may lead to delayed or ineffective recovery and disruptions to epithelial biology resulting in GI symptoms and altered barrier function (Peterson and Artis, 2014) [4]. The effect of lysozyme on the transcriptomic and proteomic profile of healthy colonic epithelial cells was investigated. Epithelial cells in culture were scratch wounded and treated with lysozyme. mRNA and protein profiles were simultaneously quantified in the same sample using a digital counting technology. Gene and protein expressions altered by the presence or absence of lysozyme are described in this article. Extensive statistical and bioinformatic analysis, and interpretation of the results can be found in "Lysozyme association with circulating RNA, extracellular vesicles, and chronic stress" (Abey et al., in press) [1].

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Source
http://dx.doi.org/10.1016/j.dib.2016.12.043DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5238358PMC
April 2017
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