For a better understanding of tumor metastasis, a reliable 3D in vitro model bridging the gap between 2D cultures and in vivo animal model studies is essential. Our study is focused on two key points: (i) designing a 3D microenvironment for studying metastasis and (ii) simulating the metastasis milieu by inducing epithelial to mesenchymal transition (EMT) and mesenchymal to epithelial transition (MET). An electrospun gelatin nanofiber matrix (EGNF) was fabricated using electrospinning and further dip coated with different concentrations of collagen to obtain surface complexity and mechanical properties, similar to connective tissues.
Our findings suggested that CCGM can be used as a tissue-like 3D model for studying breast cancer metastatic events in vitro.
Writing this article was a great pleasure as it has co-authors with whom I have had long standing collaborations. Dr. Mamatha M Pillai, PhD
Biofabrication 2017 Feb 7;9(1):015016. Epub 2017 Feb 7.
Department of Biotechnology, PSG College of Technology, Coimbatore, 641004, Tamil Nadu, India.
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