Localization and expression of molt-inhibiting hormone and nitric oxide synthase in the central nervous system of the green shore crab, Carcinus maenas, and the blackback land crab, Gecarcinus lateralis.

Authors:
Natalie L Pitts
Natalie L Pitts
Colorado State University
Donald L Mykles
Donald L Mykles
Colorado State University
United States

Comp Biochem Physiol A Mol Integr Physiol 2017 01 27;203:328-340. Epub 2016 Oct 27.

Department of Biology, Colorado State University, Fort Collins, CO 80523, USA. Electronic address:

In decapod crustaceans, molting is controlled by the pulsatile release of molt-inhibiting hormone (MIH) from neurosecretory cells in the X-organ/sinus gland (XO/SG) complex in the eyestalk ganglia (ESG). A drop in MIH release triggers molting by activating the molting gland or Y-organ (YO). Post-transcriptional mechanisms ultimately control MIH levels in the hemolymph. Neurotransmitter-mediated electrical activity controls Ca-dependent vesicular release of MIH from the SG axon terminals, which may be modulated by nitric oxide (NO). In green shore crab, Carcinus maenas, nitric oxide synthase (NOS) protein and NO are present in the SG. Moreover, C. maenas are refractory to eyestalk ablation (ESA), suggesting other regions of the nervous system secrete sufficient amounts of MIH to prevent molting. By contrast, ESA induces molting in the blackback land crab, Gecarcinus lateralis. Double-label immunofluorescence microscopy and quantitative polymerase chain reaction were used to localize and quantify MIH and NOS proteins and transcripts, respectively, in the ESG, brain, and thoracic ganglion (TG) of C. maenas and G. lateralis. In ESG, MIH- and NOS-immunopositive cells were closely associated in the SG of both species; confocal microscopy showed that NOS was localized in cells adjacent to MIH-positive axon terminals. In brain, MIH-positive cells were located in a small number of cells in the olfactory lobe; no NOS immunofluorescence was detected. In TG, MIH and NOS were localized in cell clusters between the segmental nerves. In G. lateralis, Gl-MIH and Gl-crustacean hyperglycemic hormone (CHH) mRNA levels were ~10-fold higher in ESG than in brain or TG of intermolt animals, indicating that the ESG is the primary source of these neuropeptides. Gl-NOS and Gl-elongation factor (EF2) mRNA levels were also higher in the ESG. Molt stage had little or no effect on CHH, NOS, NOS-interacting protein (NOS-IP), membrane Guanylyl Cyclase-II (GC-II), and NO-independent GC-III expression in the ESG of both species. By contrast, MIH and NO receptor GC-I beta subunit (GC-Iβ) transcripts were increased during premolt and postmolt stages in G. lateralis, but not in C. maenas. MIH immunopositive cells in the brain and TG may be a secondary source of MIH; the release of MIH from these sources may contribute to the difference between the two species in response to ESA. The MIH-immunopositive cells in the TG may be the source of an MIH-like factor that mediates molt inhibition by limb bud autotomy. The association of MIH- and NOS-labeled cells in the ESG and TG suggests that NO may modulate MIH release. A model is proposed in which NO-dependent activation of GC-I inhibits Ca-dependent fusion of MIH vesicles with the nerve terminal membrane; the resulting decrease in MIH activates the YO and the animal enters premolt.

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.cbpa.2016.10.012DOI Listing

Still can't find the full text of the article?

Sign up to send a request to the authors directly.
January 2017
37 Reads

Publication Analysis

Top Keywords

mih
14
nitric oxide
12
mih release
12
esg
8
carcinus maenas
8
green shore
8
shore crab
8
esg brain
8
land crab
8
axon terminals
8
cells
8
release mih
8
gecarcinus lateralis
8
nervous system
8
crab gecarcinus
8
blackback land
8
crab carcinus
8
higher esg
8
mrna levels
8
molt-inhibiting hormone
8

Similar Publications

Isoniazid-loaded orodispersible strips: Methodical design, optimization and in vitro-in silico characterization.

Int J Pharm 2018 Aug 5;547(1-2):347-359. Epub 2018 Jun 5.

Center for Dermal Research and Laboratory for Drug Delivery, NJ Center for Biomaterials, Life Sciences Building, Rutgers-The State University of New Jersey, 145 Bevier Road, Piscataway, NJ 08854, USA; Department of Pharmaceutics, Ernest Mario School of Pharmacy, Rutgers-The State University of New Jersey, Piscataway, NJ 08854, USA. Electronic address:

Drug treatment remains the most effective global approach to managing and preventing tuberculosis. This work focuses on formulating and evaluating an optimized polyvinyl alcohol-polyethylene glycol based orodispersible strip containing isoniazid, a first-line anti-tubercular agent. A solvent casting method guided through a Taguchi experimental design was employed in the fabrication, optimization and characterization of the orodispersible strip. Read More

View Article
August 2018
51 Reads
3.65 Impact Factor

Overall treatment duration with multimodality treatment approach and outcome results in oral cavity carcinoma: A study from an institute from central India.

J Cancer Res Ther 2017 Apr-Jun;13(2):351-355

Department of Microbiology, Rural Medical College, Loni Bk, Maharashtra, India.

Objective Of The Study: To see the different age groups, gender, sites, disease stage, treatment outcome of various oral cavity sites carcinoma by combined modalities and the overall treatment duration.

Materials And Methods: A total of 212 oral cavity carcinoma patients (169 males and 43 females) with complete records (from September 2009 to December 2012) were analyzed for age, sex, histopathology, associated medical illnesses, various subsites with disease stage, various treatment modalities with the duration and follow-up records for disease control as well as disease failure at local, nodal, local + nodal, and distant metastasis.

Results: The most common site in oral cavity cancer was buccal mucosa 81 (38. Read More

View Article
April 2018
30 Reads

Synergic phototoxic effect of visible light or Gallium-Arsenide laser in the presence of different photo-sensitizers on Porphyromonas gingivalis and Fusobacterium nucleatum.

Dent Res J (Isfahan) 2015 Jul-Aug;12(4):323-30

Dental Implant Research Center and Oral and Maxillofacial Surgery, School of Dentistry, Isfahan University of Medical Sciences, Isfahan, Iran.

Background: According to the development of resistant strains of pathogenic bacteria following treatment with antimicrobial chemotherapeutic agents, alternative approaches such as lethal photosensitization are being used. The aim of this study was to evaluate the effect of visible light and laser beam radiation in conjugation with three different photosensitizers on the survival of two main periodontopathogenic bacteria including Porphyromonas gingivalis and Fusobacterium nucleatum in different exposure periods.

Materials And Methods: In this in vitro prospective study, strains of P. Read More

View Article
August 2015
25 Reads

Carum copticum L.: a herbal medicine with various pharmacological effects.

Biomed Res Int 2014 25;2014:569087. Epub 2014 Jun 25.

Neurogenic Inflammation Research Centre and Department of Physiology, School of Medicine, Mashhad University of Medical Sciences, Mashhad 9177948564, Iran.

Carum copticum L. commonly known as "Ajwain" is cultivated in many regions of the world including Iran and India, states of Gujarat and Rajasthan. Traditionally, C. Read More

View Article
September 2015
5 Reads
1 PubMed Central Citation(source)

Soluble vascular endothelial growth factor receptor 1 in tracheal aspirate fluid of preterm neonates at birth may be predictive of bronchopulmonary dysplasia/chronic lung disease.

Pediatrics 2009 Jun;123(6):1541-7

Division of Neonatal-Perinatal Medicine, Department of Pediatrics, University of California Irvine Medical Center, 101 The City Drive South, Route 81, Building 56, Suite 600, Orange, CA 92868, USA.

Objective: We tested the hypothesis that soluble vascular endothelial growth factor receptors are involved in the development of bronchopulmonary dysplasia/chronic lung disease.

Patients And Methods: Neonates with a birth weight of < or =1500 g and/or < or =30 weeks' gestation, with respiratory failure, requiring O(2) and mechanical ventilation within 24 hours, were eligible. Tracheal aspirate fluid samples were collected from 65 neonates before surfactant and/or assisted mechanical ventilation (baseline), at 3 and 7 days after birth, and weekly thereafter until extubation. Read More

View Article
June 2009
10 Reads
10 PubMed Central Citations(source)
5.47 Impact Factor