Immunochromatographic determination of bacopaside I in biological samples.

J Chromatogr B Analyt Technol Biomed Life Sci 2017 Jan 23;1040:60-66. Epub 2016 Nov 23.

Department of Pharmaceutical Chemistry and Pharmacognosy, Faculty of Pharmaceutical Sciences and Center of Excellence for Innovation in Chemistry, Naresuan University, Phitsanulok 65000, Thailand. Electronic address:

We describe a novel immunochromatographic method for qualitative and quantitative analyses of bacopaside I, a bioactive constituent in Bacopa monnieri (L.) Wettst in biological samples. The assay was performed on polyethersulfone membrane using a polyclonal antibody raised against bacopaside I. The finalised method could quantitatively determine bacopaside I in the range of 31.3-1000.0ng and the detection and quantification limits were 1.0 and 31.3ng, respectively. The percentage recoveries of bacopaside I in blood and urine were nearly 100% indicating the accuracy of the extraction. The method was then applied for the determination of this compound in rat serum, urine and feces after an oral dose of 15mg/kg body weight. About 4% of the ingested dose of bacopaside I was detected in rat feces but none was detected in serum and urine which accorded with results from liquid chromatography tandem mass spectrometry. The accuracy, selectivity, sensitivity of the method are appropriate for in vivo pharmacokinetic studies.

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jchromb.2016.11.022DOI Listing
January 2017

Publication Analysis

Top Keywords

biological samples
8
serum urine
8
bacopaside
6
extraction method
4
method applied
4
accuracy extraction
4
indicating accuracy
4
applied determination
4
compound rat
4
feces oral
4
urine feces
4
rat serum
4
100% indicating
4
determination compound
4
blood urine
4
313-10000ng detection
4
detection quantification
4
range 313-10000ng
4
bacopaside range
4
determine bacopaside
4

Similar Publications