GEN3VA: aggregation and analysis of gene expression signatures from related studies.

BMC Bioinformatics 2016 Nov 15;17(1):461. Epub 2016 Nov 15.

Department of Pharmacological Sciences, One Gustave L. Levy Place, Box 1603, New York, NY, 10029, USA.

Background: Genome-wide gene expression profiling of mammalian cells is becoming a staple of many published biomedical and biological research studies. Such data is deposited into data repositories such as the Gene Expression Omnibus (GEO) for potential reuse. However, these repositories currently do not provide simple interfaces to systematically analyze collections of related studies.

Results: Here we present GENE Expression and Enrichment Vector Analyzer (GEN3VA), a web-based system that enables the integrative analysis of aggregated collections of tagged gene expression signatures identified and extracted from GEO. Each tagged collection of signatures is presented in a report that consists of heatmaps of the differentially expressed genes; principal component analysis of all signatures; enrichment analysis with several gene set libraries across all signatures, which we term enrichment vector analysis; and global mapping of small molecules that are predicted to reverse or mimic each signature in the aggregate. We demonstrate how GEN3VA can be used to identify common molecular mechanisms of aging by analyzing tagged signatures from 244 studies that compared young vs. old tissues in mammalian systems. In a second case study, we collected 86 signatures from treatment of human cells with dexamethasone, a glucocorticoid receptor (GR) agonist. Our analysis confirms consensus GR target genes and predicts potential drug mimickers.

Conclusions: GEN3VA can be used to identify, aggregate, and analyze themed collections of gene expression signatures from diverse but related studies. Such integrative analyses can be used to address concerns about data reproducibility, confirm results across labs, and discover new collective knowledge by data reuse. GEN3VA is an open-source web-based system that is freely available at: http://amp.pharm.mssm.edu/gen3va .

Download full-text PDF

Source
http://dx.doi.org/10.1186/s12859-016-1321-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5111283PMC
November 2016
8 Reads
2.576 Impact Factor

Publication Analysis

Top Keywords

gene expression
24
expression signatures
12
gen3va identify
8
signatures
8
enrichment vector
8
analysis gene
8
web-based system
8
gene
7
analysis
6
expression
6
gen3va
5
identify common
4
common molecular
4
signature aggregate
4
mimic signature
4
reverse mimic
4
molecular mechanisms
4
aggregate demonstrate
4
demonstrate gen3va
4
signatures 244
4

References

(Supplied by CrossRef)

T Barrett et al.
Nucleic Acids Res 2013

A Brazma et al.
Nucleic Acids Res 2003

SA Ochsner et al.
Cancer Res 2009

K Kerkentzes et al.
Front Oncol 2014

Y Zhu et al.
Bioinformatics 2008

S Davis et al.
Bioinformatics 2007

GW Gundersen et al.
Bioinformatics 2015

NR Clark et al.
BMC Bioinformatics 2014

Similar Publications