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Differential expression of gene in two cultivars under water deficit stress.

Authors:
Bahlanes Bakhtari Hooman Razi

Mol Biol Res Commun 2014 Dec;3(4):241-251

Department of Crop Production and Plant Breeding, College of Agriculture, Shiraz University, Shiraz, Iran.

The sucrose non-fermenting 1-related protein kinase 2 (SnRK2) family members are plant unique serine/threonine kinases which play a key role in cellular signaling in response to abiotic stresses. The three SnRK2 members including SRK2D, SRK2I and SRK2E are known to phosphorylate major abscisic acid (ABA) responsive transcription factors, ABF2 and ABF4, involved in an ABA-dependent stress signaling pathway in Arabidopsis. This study aimed to clone and sequence an ortholog of the Arabidopsis gene from , designated as . An 833bp cDNA fragment of , which shared high amino acid sequence identity with its Arabidopsis counterpart, was obtained suggesting a possible conserved function for these genes. The expression pattern of and its potential target gene ABF2 () were then analyzed in the two cultivars with contrasting reaction to water deficit stress. Semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) showed that and were water-deficit stress responsive genes with similar expression profiles. The accumulation of the and transcripts in the two cultivars was linked with their level of drought tolerance, as the drought tolerant cultivar had significantly higher expression levels of both genes under normal and water deficit stress conditions. These findings suggest that and genes may be involved in conferring drought tolerance in .

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5019310PMC
December 2014

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