Silk–PVA Hybrid Nanofibrous Scaffolds for Enhanced Primary Human Meniscal Cell Proliferation

Mamatha M Pillai, J Gopinathan, B Indumathi, Y R Manjoosha, K Santosh Sahanand, B K Dinakar Rai, R Selvakumar, Amitava Bhattacharyya

Overview

In this study, silk fibroin nanofibrous scaffolds were developed to investigate the attachment and proliferation of primary human meniscal cells. Silk fibroin (SF)–polyvinyl alcohol (PVA) blended electrospun nanofibrous scaffolds with different blend ratios (2:1, 3:1, and 4:1) were prepared.

Summary

A novel composite suitable for knee meniscus tissue engineering

Author Comments

Dr. Mamatha M Pillai, PhD
Dr. Mamatha M Pillai, PhD
Indian Institute of Technology, Bombay
Post Doctoral Fellow
Tissue engineering and regenerative medicine
Mumbai, Maharashtra | India
I hope you find this article thought-provoking.Dr. Mamatha M Pillai, PhD

Resources

PUBMED
https://link.springer.com/article/10.1007/s00232-016-9932-z

Silk-PVA Hybrid Nanofibrous Scaffolds for Enhanced Primary Human Meniscal Cell Proliferation.

Authors:
Dr. Mamatha M Pillai, PhD
Dr. Mamatha M Pillai, PhD
Indian Institute of Technology, Bombay
Post Doctoral Fellow
Tissue engineering and regenerative medicine
Mumbai, Maharashtra | India

J Membr Biol 2016 12 11;249(6):813-822. Epub 2016 Oct 11.

Advanced Textile and Polymer Research Laboratory, PSG Institute of Advanced Studies, Coimbatore, 641004, India.

In this study, silk fibroin nanofibrous scaffolds were developed to investigate the attachment and proliferation of primary human meniscal cells. Silk fibroin (SF)-polyvinyl alcohol (PVA) blended electrospun nanofibrous scaffolds with different blend ratios (2:1, 3:1, and 4:1) were prepared. Morphology of the scaffolds was characterized using atomic force microscopy (AFM). The hybrid nanofibrous mats were crosslinked using 25 % (v/v) glutaraldehyde vapor. In degradation study, the crosslinked nanofiber showed slow degradation of 20 % on weight after 35 days of incubation in simulated body fluid (SBF). The scaffolds were characterized with suitable techniques for its functional groups, porosity, and swelling ratio. Among the nanofibers, 3:1 SF:PVA blend showed uniform morphology and fiber diameter. The blended scaffolds had fluid uptake and swelling ratio of 80 % and 458 ± 21 %, respectively. Primary meniscal cells isolated from surgical debris after meniscectomy were subcultured and seeded onto these hybrid nanofibrous scaffolds. Meniscal cell attachment studies confirmed that 3:1 SF:PVA nanofibrous scaffolds supported better cell attachment and growth. The DNA and collagen content increased significantly with 3:1 SF:PVA. These results clearly indicate that a blend of SF:PVA at 3:1 ratio is suitable for meniscus cell proliferation when compared to pure SF-PVA nanofibers.

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http://dx.doi.org/10.1007/s00232-016-9932-zDOI Listing
December 2016
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