Fully automated, clinical-grade bone marrow processing: a single-centre experience.

Blood Transfus 2017 Oct 27;15(6):577-584. Epub 2016 Sep 27.

Cell Therapy and Transfusion Medicine Unit, Cord Blood Bank, Careggi University Hospital, Florence, Italy.

Background: Clinical grade processing of harvested bone marrow is required in various clinical situations, particularly in the management of ABO mismatching in allogeneic haematopoietic stem cell transplantation (HSCT) and in regenerative medicine.

Material And Methods: We report a single-centre experience using a fully automated, clinical grade, closed system (Sepax, Biosafe, Switzerland). From 2003 to 2015, 125 procedures were performed in our laboratory, including buffy-coat production for HSCT (n=58), regenerative medicine in an orthopaedic setting (n=54) and density-gradient separation in a trial for treatment of critical limb ischaemia (n=13).

Results: Buffy coat separation resulted in a median volume reduction of 85% (range, 75-87%), providing satisfactory red blood cell depletion (69%, range 30-88%) and a median recovery of CD34 cells of 96% (range, 81-134%) in the setting of allogeneic HSCT. Significantly greater volume reduction (90%; range, 90-92%) and red blood cell depletion (88%; range, 80-93%) were achieved by the new SmartRedux software released for Sepax2, validated in the last eight allogeneic HSCT. The density gradient separation programme resulted in complete red blood cell depletion associated with high CD34 recovery (69%; range, 36-124%). No reactions related to the quality of the product were reported. Time to engraftment following allogeneic HSCT was in the normal range. No cases of microbiological contamination related to the manipulation were reported.

Discussion: Clinical grade, automated bone marrow manipulation with Sepax was shown to be effective, giving operator-independent results and could be used for a broad range of clinical applications.

Download full-text PDF

Source
http://www.bloodtransfusion.it/articolo.aspx?idart=003092&am
Publisher Site
http://dx.doi.org/10.2450/2016.0057-16DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5649968PMC
October 2017

Publication Analysis

Top Keywords

cell depletion
12
bone marrow
12
allogeneic hsct
12
blood cell
12
clinical grade
12
red blood
12
range
8
volume reduction
8
69% range
8
single-centre experience
8
fully automated
8
clinical
5
hsct
5
density-gradient separation
4
n=54 density-gradient
4
limb ischaemia
4
setting n=54
4
validated allogeneic
4
achieved smartredux
4
smartredux software
4

Similar Publications