Fully automated chemiluminescence detection using an electrified-Lab-on-a-Disc (eLoaD) platform.

Niamh A Kilcawley
Niamh A Kilcawley
Biomedical Diagnostics Institute
Dr. Jens Ducree, Dr. rer. nat. habil. Dipl. Phys.
Dr. Jens Ducree, Dr. rer. nat. habil. Dipl. Phys.
Fraunhofer Project Centre at Dublin City University
Professor (Full)
microfluidics, Lab-on-a-Chip, hydrodynanmics, business development, project management, organisational leadership
Glasnevin, Dublin 9 | Ireland
Dario Mager
Dario Mager
University of Freiburg

Lab Chip 2016 10;16(20):4002-4011

Institute of Microstructure Technology, Karlsruhe Institute of Technology, Hermann-von-Helmholtz-Platz 1, Eggenstein-Leopoldshafen 76344, Germany.

Typical Lab-on-a-Disc (LoaD) platforms cannot make a continuous measurement while the disc is spinning; this drawback means that the disc usually must be stopped and aligned with a sensor. This can result in measurement errors in time-dependent assays along with inaccuracies due to liquid displacement and bubble formation in the absence of a stabilising centrifugal field. This paper presents a novel concept for a wirelessly electrified-Lab-on-a-Disc (eLoaD) platform that allows continuous measurement of experimental parameters while the disc is spinning. This platform incorporates all the components needed for measurement within the rotating frame of reference, and bidirectional transmission of data outside this reference frame, thus allowing for online measurement independent of the rotation of the disc. The eLoaD platform is conceived in a modular manner whereby an interchangeable and non-disposable 'Application Disc' can be fitted to the eLoaD platform and so the system can be adapted for a range of optical, electrochemical and other measurement types. As an application example, optical readout, using the Application Disc fitted with a silicon photomultiplier, is demonstrated using a tagged chemiluminescent antibody, which is commonly used, for instance, in ELISA assays. The precision of the eLoaD platform is >94%, while its accuracy, when compared to a commercial benchtop luminometer, is higher than 96%. The modular design of this platform will permit extension of this technology to many other LoaD applications.

Download full-text PDF

http://dx.doi.org/10.1039/c6lc00973eDOI Listing

Still can't find the full text of the article?

Sign up to send a request to the authors directly.
October 2016
18 Reads

Publication Analysis

Top Keywords

eload platform
electrified-lab-on-a-disc eload
disc spinning
continuous measurement
modular manner
manner interchangeable
platform conceived
disc eload
interchangeable non-disposable
conceived modular
disc' fitted
adapted range
range optical
optical electrochemical
electrochemical measurement
system adapted


(Supplied by CrossRef)
Article in J. Lab. Autom.
Kong et al.
J. Lab. Autom. 2016
Article in J. Micromech. Microeng.
Ducrée et al.
J. Micromech. Microeng. 2007
Article in Chem. Soc. Rev.
Strohmeier et al.
Chem. Soc. Rev. 2015
Article in PLoS One
Kinahan et al.
PLoS One 2016
Article in Sens. Actuators, A
Kinahan et al.
Sens. Actuators, A 2014
Article in Lab Chip
Gorkin et al.
Lab Chip 2010
Article in Anal. Chem.
Kim et al.
Anal. Chem. 2014
Article in Biosens. Bioelectron.
Nwankire et al.
Biosens. Bioelectron. 2015
Article in Anal. Chem.
Park et al.
Anal. Chem. 2014
Article in Anal. Chem.
Hwang et al.
Anal. Chem. 2013
Article in Sens. Actuators, B
Amasia et al.
Sens. Actuators, B 2012

Similar Publications

A Cross-Sectional Study to Assess and Correlate Osteoporosis and Periodontitis among Postmenopausal Women: A Dual Energy X-Ray Absorptiometry Study.

J Midlife Health 2018 Jan-Mar;9(1):2-7

Department of Periodontology, School of Dental Sciences, Krishna Institute of Medical Sciences Deemed University, Karad, Maharashtra, India.

Background: Periodontitis and osteoporosis are two diseases found worldwide and increase in intensity with increasing age of the patients. The severity of osteoporosis and periodontitis are found to be more in women during menopause. The aim of this study was to assess and correlate osteoporosis and periodontal disease among post-menopausal women. Read More

View Article
April 2018
15 Reads

Effect of combined treatment with focused mechano-acoustic vibration and pharmacological therapy on bone mineral density and muscle strength in post-menopausal women.

Clin Cases Miner Bone Metab 2017 Sep-Dec;14(3):305-311. Epub 2017 Dec 27.

Associated Professor, Physical and Rehabilitation Medicine, Department of Medicine and Sciences of Aging, "Gabriele d'Annunzio" University of Chieti-Pescara, Italy.

Introduction: Osteoporosis is a systemic disease of the skeleton characterized by a reduction in bone mass and alterations in microarchitecture accompanied by increase in fracture risk, with a relevant decline in quality of life and important social, economic, and health implications, representing one of the most common causes of disability and a major financial item of health cost in many Countries. The best therapy for osteoporosis is prevention, consisting in measures to avoid or slow the onset of the disease. Treatment includes measures aimed at osteoporotic individuals, with or without previous fractures and a high risk of a first or additional fracture. Read More

View Article
December 2017
12 Reads

Ultrashort single-walled carbon nanotubes in a lipid bilayer as a new nanopore sensor.

Nat Commun 2013 ;4:2989

Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety, Institute of High Energy Physics, Chinese Academy of Sciences, 19B Yuquan Road, Beijing 100049, China.

An important issue in nanopore sensing is to construct stable and versatile sensors that can discriminate analytes with minute differences. Here we report a means of creating nanopores that comprise ultrashort single-walled carbon nanotubes inserted into a lipid bilayer. We investigate the ion transport and DNA translocation through single-walled carbon nanotube nanopores and find that our results are fundamentally different from previous studies using much longer single-walled carbon nanotubes. Read More

View Article
July 2014
9 Reads
15 PubMed Central Citations(source)
10.74 Impact Factor

Dexmedetomidine-saves the day.

Indian J Anaesth 2013 Mar;57(2):212-3

Department of Anaesthesiology, Saifee Hospital, Charni Road, Mumbai, Maharashtra, India.

View Article
March 2013
11 Reads


Acta Crystallogr Sect E Struct Rep Online 2009 Jun 27;65(Pt 7):i55. Epub 2009 Jun 27.

Dinickel(II) silicon bis-[diphosphate(4-)], Ni(2)Si(P(2)O(7))(2), is isotypic with other phosphates of the formula M(2)Si(P(2)O(7))(2) (M = Co, Cd). All atoms except Si (site symmetry 2) are found in general positions. Ni(2)O(10) dimers formed from edge-sharing NiO(6) octa-hedra are linked by corners and O-P-O bridges, forming slabs parallel to (100), which are in turn inter-connected by O-Si-O contacts. Read More

View Article
June 2009
12 Reads