Antimicrobial susceptibility and vaginolysin in Gardnerella vaginalis from healthy and bacterial vaginosis diagnosed women.

J Infect Dev Ctries 2016 Sep 30;10(9):913-919. Epub 2016 Sep 30.

Federal University of Juiz de Fora, Juiz de Fora, Minas Gerais, Brazil.

Introduction: Bacterial vaginosis (BV) is a syndrome related to Gardnerella vaginalis and is characterized by an imbalance in the vaginal microbiota. This work focused on the evaluation of antimicrobial susceptibility patterns and the occurrence of the vaginolysin (vly) gene in G. vaginalis isolated from BV and non-BV patients.

Methodology: The vaginal secretions were collected randomly and processed for G. vaginalis isolation. The isolates were presumptively identified by β-hemolysis and oxidase and catalase tests. Polymerase chain reaction (PCR) was performed to confirm bacterial identity and to detect the vly gene. Antimicrobial susceptibility patterns were determined.

Results: Of 89 patients, G. vaginalis was isolated from 42 (37 BV and 5 non-BV), and 204 isolates were selected (179 from BV and 25 non-BV). The vly gene was detected in all G. vaginalis isolated from non-BV women and in 98.3% of the bacteria from BV patients. High resistance was observed for ampicillin (54.4%), metronidazole (59.8%), tinidazole (60.3%) and secnidazole (71.6%).

Conclusions: Further studies are needed to better address the role of G. vaginalis and the vly gene in BV pathogenesis.

Download full-text PDF

Source
http://dx.doi.org/10.3855/jidc.7161DOI Listing
September 2016
44 Reads

Publication Analysis

Top Keywords

vly gene
16
isolated non-bv
12
vaginalis isolated
12
antimicrobial susceptibility
12
gardnerella vaginalis
8
bacterial vaginosis
8
susceptibility patterns
8
vaginalis
7
identity detect
4
bacterial identity
4
performed confirm
4
confirm bacterial
4
gene antimicrobial
4
non-bv 204
4
204 isolates
4
patients vaginalis
4
determinedresults patients
4
pcr performed
4
patterns determinedresults
4
detect vly
4

Similar Publications