Functional and structural changes of the urinary bladder following spinal cord injury; treatment with alpha lipoic acid.

Neurourol Urodyn 2017 04 4;36(4):1061-1068. Epub 2016 Aug 4.

Department of Pharmacology and Therapeutics, University of Florida College of Medicine, Gainesville, Florida.

Background & Aim: Alpha lipoic acid (LA) was shown to exert neuroprotection in trauma-induced spinal cord injury (SCI), which is frequently associated with urinary bladder complaints in patients with SCI. Accordingly, the protective effects of LA on biochemical and histological changes in bladder as well as functional studies were assessed.

Methods: Wistar albino rats were divided as control, SCI, and LA (50 mg/kg/day, ip) treated SCI groups (SCI+LA). The standard weight-drop (100 g/cm force at T10) method was used to induce a moderately severe SCI. One week after the injury, neurological examination was performed and the rats were decapitated. Bladder samples were taken for histological examination, functional (isolated tissue bath) studies, and for the measurement of biochemical parameters (malondialdehyde, MDA; gluthathione, GSH; nerve growth factor, NGF; caspase-3, luminol and lucigenin chemiluminescences).

Results: SCI caused a significant (P < 0.001) increase in the detrusor muscle thickness. It increased the contractility responses to carbachol and relaxation responses to papaverine (P < 0.05-0.001). There were also significant alterations in MDA, caspase-3, luminol, and lucigenin chemiluminescences with concomitant decreases in NGF and GSH (P < 0.05). LA treatment reversed histological and functional (contraction and relaxation responses) changes induced by SCI (P < 0.05-0.001), but no significant recovery was observed in the impaired neurological functions.

Conclusion: These results indicate that LA have a beneficial effect in improving the bladder tonus via its antioxidant and anti-inflammatory actions following SCI.

Download full-text PDF

Source
http://dx.doi.org/10.1002/nau.23083DOI Listing
April 2017
27 Reads
2.873 Impact Factor

Publication Analysis

Top Keywords

urinary bladder
8
spinal cord
8
cord injury
8
lipoic acid
8
alpha lipoic
8
sci
6
induce moderately
4
t10 method
4
method induce
4
moderately severe
4
sci week
4
examination performed
4
performed rats
4
neurological examination
4
injury neurological
4
force t10
4
week injury
4
severe sci
4
weight-drop 100 g/cm
4
divided control
4

Similar Publications