In the present study, recombinant plasmid pET22b? was transformed into E. coli strains BL21(DE3) and RosettaTM(DE3). The SDS-PSAGE analysis showed no CPB band from E. coli/BL21/ pET22? but showed a CPB band from E. coli/RosettaTM/pET22?. The sequencing analysis of purified pET22? revealed that the inserted gene had 987 bp, demonstrating a 99% – 100% identity with cpb genes that were previously deposited in GenBank. The recombinant toxin was expressed 30 min after induction with IPTG and continued for 18 h.
pET22b(+) and E. coli strain RosettaTM(DE3) are suitable expression vectors and hosts that can enhance the expression and production of C. perfringens recombinant beta toxin. Therefore, the E. coli/RosettaTM/pET22? clone could be used for further research on recombinant vaccine production.
Iranian variant type B was isolated in 1954 from intestinal contents of sheep and goats. The three strains of Clostridium welchii type B isolated were different from the classical typeB strains in their production of kappa and non-production of lambda and hyaluronidase toxins. Two of the strains were isolated from young goats and the other from an adult sheepDr. Reza Pilehchian Langroudi, PhD
Onderstepoort J Vet Res 2016 Jun 30;83(1):a1136. Epub 2016 Jun 30.
Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Alborz, Karaj.
Download full-text PDF