Search our Database of Scientific Publications and Authors

I’m looking for a
    Investigating strain dependency in the production of aromatic compounds in Saccharomyces cerevisiae.
    Biotechnol Bioeng 2016 Dec 30;113(12):2676-2685. Epub 2016 Jun 30.
    Department of Chemical and Biological Engineering, Iowa State University, Ames, Iowa.
    Although Saccharomyces cerevisiae is the most highly domesticated yeast, strain dependency in biotechnological processes still remains as a common, yet poorly understood phenomenon. To investigate this, the entrance to the aromatic amino acid biosynthetic pathway was compared in four commonly used S. cerevisiae laboratory strains. The strains were engineered to accumulate shikimate by overexpressing a mutant version of the pentafunctional ARO1 enzyme with disrupted activity in the shikimate kinase subunit. Carbon tracing and (13) C metabolic flux analysis combined with quantitative PCR, revealed that precursor availability and shikimate production were dramatically different in the four equally engineered strains, which were found to be correlated with the strains' capacity to deal with protein overexpression burden. By implementing a strain-dependent approach, the genetic platform was reformulated, leading to an increase in yield and titer in all strains. The highest producing strain, INVSc1-SA3, produced 358 mg L(-1) of shikimate with a yield of 17.9 mg g(-1)glucose. These results underline the importance of strain selection in developing biological manufacturing processes, demonstrate the first case of high production of shikimate in yeast, and provide an appropriate platform for strain selection for future production of aromatic compounds. Biotechnol. Bioeng. 2016;113: 2676-2685. © 2016 Wiley Periodicals, Inc.

    Similar Publications

    Production of para-aminobenzoic acid from different carbon-sources in engineered Saccharomyces cerevisiae.
    Microb Cell Fact 2016 May 26;15:89. Epub 2016 May 26.
    Centre for Microbial Electrochemical Systems (CEMES), The University of Queensland, Office 618, Level 6 Gehrmann Building (60), St. Lucia, Brisbane, QLD, 4072, Australia.
    Background: Biological production of the aromatic compound para-aminobenzoic acid (pABA) is of great interest to the chemical industry. Besides its application in pharmacy and as crosslinking agent for resins and dyes pABA is a potential precursor for the high-volume aromatic feedstocks terephthalic acid and para-phenylenediamine. The yeast Saccharomyces cerevisiae synthesises pABA in the shikimate pathway: Outgoing from the central shikimate pathway intermediate chorismate, pABA is formed in two enzyme-catalysed steps, encoded by the genes ABZ1 and ABZ2. Read More
    Plasmid-encoded biosynthetic genes alleviate metabolic disadvantages while increasing glucose conversion to shikimate in an engineered Escherichia coli strain.
    Biotechnol Bioeng 2017 Jun 9;114(6):1319-1330. Epub 2017 Mar 9.
    Instituto de Biotecnología, Universidad Nacional Autónoma de México (UNAM), Cuernavaca, Morelos, Mexico.
    Metabolic engineering strategies applied over the last two decades to produce shikimate (SA) in Escherichia coli have resulted in a battery of strains bearing many expression systems. However, the effects that these systems have on the host physiology and how they impact the production of SA are still not well understood. In this work we utilized an engineered E. Read More
    Metabolic engineering of a tyrosine-overproducing yeast platform using targeted metabolomics.
    Microb Cell Fact 2015 May 28;14:73. Epub 2015 May 28.
    Department of Biology and Centre for Structural and Functional Genomics, Concordia University, 7141 Sherbrooke West, Montreal, QC, H4B 1R6, Canada.
    Background: L-tyrosine is a common precursor for a wide range of valuable secondary metabolites, including benzylisoquinoline alkaloids (BIAs) and many polyketides. An industrially tractable yeast strain optimized for production of L-tyrosine could serve as a platform for the development of BIA and polyketide cell factories. This study applied a targeted metabolomics approach to evaluate metabolic engineering strategies to increase the availability of intracellular L-tyrosine in the yeast Saccharomyces cerevisiae CEN. Read More
    Iterative optimization of xylose catabolism in Saccharomyces cerevisiae using combinatorial expression tuning.
    Biotechnol Bioeng 2017 Jun 20;114(6):1301-1309. Epub 2017 Feb 20.
    Department of Bioengineering, University of California, 2151 Berkeley Way, Berkeley, California 94720.
    A common challenge in metabolic engineering is rapidly identifying rate-controlling enzymes in heterologous pathways for subsequent production improvement. We demonstrate a workflow to address this challenge and apply it to improving xylose utilization in Saccharomyces cerevisiae. For eight reactions required for conversion of xylose to ethanol, we screened enzymes for functional expression in S. Read More