Rapidly Probing Antibacterial Activity of Graphene Oxide by Mass Spectrometry-based Metabolite Fingerprinting.

Sci Rep 2016 06 16;6:28045. Epub 2016 Jun 16.

Beijing National Laboratory for Molecular Sciences, Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry Chinese Academy of Sciences, Beijing 100190, China.

Application of nanomaterials as anti-bacteria agents has aroused great attention. To investigate the antibacterial activity and antibacterial mechanism of nanomaterials from a molecular perspective is important for efficient developing of nanomaterial antibiotics. In the current work, a new mass spectrometry-based method was established to investigate the bacterial cytotoxicity of graphene oxide (GO) by the metabolite fingerprinting of microbes. The mass spectra of extracted metabolites from two strains DH5α and ATCC25922 were obtained before and after the incubation with nanomaterials respectively. Then principal component analysis (PCA) of these spectra was performed to reveal the relationship between the metabolism disorder of microbes and bactericidal activity of GO. A parameter "D" obtained from PCA scores was proposed that is capable to quantitatively evaluate the antibacterial activity of GO in concentration and time-dependent experiments. Further annotation of the fingerprinting spectra shows the variabilities of important metabolites such as phosphatidylethanolamine, phosphatidylglycerol and glutathione. This metabolic perturbation of E. coli indicates cell membrane destruction and oxidative stress mechanisms for anti-bacteria activity of graphene oxide. It is anticipated that this mass spectrometry-based metabolite fingerprinting method will be applicable to other antibacterial nanomaterials and provide more clues as to their antibacterial mechanism at molecular level.

Download full-text PDF

Source
http://dx.doi.org/10.1038/srep28045DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4910068PMC
June 2016
41 Reads

Publication Analysis

Top Keywords

graphene oxide
12
antibacterial activity
12
mass spectrometry-based
12
metabolite fingerprinting
12
activity graphene
8
spectrometry-based metabolite
8
antibacterial mechanism
8
antibacterial
6
activity
5
strains dh5α
4
metabolites strains
4
glutathione metabolic
4
phosphatidylethanolamine phosphatidylglycerol
4
phosphatidylglycerol glutathione
4
atcc25922 incubation
4
variabilities metabolites
4
nanomaterials principal
4
incubation nanomaterials
4
extracted metabolites
4
metabolites phosphatidylethanolamine
4

References

(Supplied by CrossRef)

K Blecher et al.
Virulence 2011

Y Zhao et al.
Nanoscale 2013

Y Zhao et al.
J. Am. Chem. Soc. 2013

WR Li et al.
Appl. Microbiol. Biot. 2010

L Cui et al.
Anal. Chem. 2013

KR Raghupathi et al.
Langmuir 2011

Y Liu et al.
J. Appl. Microbiol. 2009

M Planchon et al.
Colloid. Surface. B 2013

DY Lyon et al.
Environ. Sci. Technol. 2006

J Wehling et al.
ACS Nano 2014

HQ Chen et al.
Small 2013

Similar Publications