PLoS One 2016 8;11(6):e0156754. Epub 2016 Jun 8.
Department of Chemistry and Biology, Ryerson University, Toronto, Ontario, Canada.
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BMC Biotechnol 2017 05 18;17(1):45. Epub 2017 May 18.
Department of Physics, Ryerson University, 350 Victoria Street Toronto, Ontario, M5B 2K3, Canada.
Background: Ultrasound and microbubbles (USMB) have been shown to enhance the intracellular uptake of molecules, generally thought to occur as a result of sonoporation. The underlying mechanism associated with USMB-enhanced intracellular uptake such as membrane disruption and endocytosis may also be associated with USMB-induced release of cellular materials to the extracellular milieu. This study investigates USMB effects on the molecular release from cells through membrane-disruption and exocytosis. Read More
Int J Mol Sci 2017 Jul 25;18(8). Epub 2017 Jul 25.
Imaging Division, University Medical Center Utrecht, Utrecht, 3584 CX, The Netherland.
Microbubbles-assisted ultrasound (USMB) has shown promise in improving local drug delivery. The formation of transient membrane pores and endocytosis are reported to be enhanced by USMB, and they contribute to cellular drug uptake. Exocytosis also seems to be linked to endocytosis upon USMB treatment. Read More
Mol Pharm 2014 Aug 26;11(8):2887-95. Epub 2014 Jun 26.
Fischell Department of Bioengineering, University of Maryland , College Park, Maryland 20742-4450, United States.
Drugs often use endocytosis to achieve intracellular delivery, either by passive uptake from the extracellular fluid or by active targeting of cell surface features such as endocytic receptors. An example is enzyme replacement therapy, a clinically practiced treatment for several lysosomal storage diseases where glycosylated recombinant enzymes naturally target the mannose-6-phosphate receptor and are internalized by clathrin mediated endocytosis (CME). However, lysosomal substrate accumulation, a hallmark of these diseases, has been indirectly linked to aberrant endocytic activity. Read More
Circ Res 2009 Mar 22;104(5):679-87. Epub 2009 Jan 22.
Department of Clinical Pharmacology, University Medical Center Groningen, University of Groningen, The Netherlands.
Contrast microbubbles in combination with ultrasound (US) are promising vehicles for local drug and gene delivery. However, the exact mechanisms behind intracellular delivery of therapeutic compounds remain to be resolved. We hypothesized that endocytosis and pore formation are involved during US and microbubble targeted delivery (UMTD) of therapeutic compounds. Read More